摘要
目的进一步认识丙型肝炎病毒（ｈｅｎａｔｉｔｉｓ Ｃ ｖｉｒｕｓ， ＨＣＶ）包膜糖蛋白高变区１（ｈｙｎｅｒｎａｒｉｂｌｅ ｒｅ－ｇｉｏｎ １，ＨＶＲ１）序列的抗原表位。方法设计并台成ＨＣＶ－ＢＪ株（１ｂ型）ＨＶＲ１ ３９０－４１１序列共２２个氨基酸的线性表位多肽（ＬＰ）及多抗原肽（ＭＡＰ），并比较与ＨＣＶ感染者血清中抗ＨＶＲ１的反应性。结果抗ＭＡＰ的Ａ４５０为０．４６１±０．５９０，抗ＬＰ为０．１４５±０．１６６，两者差异有非常显著性（Ｐ＜０．０００１），而且两者有较好的关联性（Ｐ＜ ０００１）；５９份抗ＨＣＶ阳性血清中，抗ＬＰ及抗ＭＡＰ的阳性检出率分别为２９％和５８％；ＭＡＰ可结合ＨＣＶ １ｂ型以外其它基因型ＨＣＶ感染者血清中相应的抗体。结论ＭＡＰ与天然抗体结合的反应原性明显高于ＬＰ，且存在一定的交叉反应性；合成合ＨＣＶ ＨＶＲ１序列的ＭＡＰ可能成为分析相应区段构型抗原表位和设计ＨＣＶ分子疫苗一条可行的途径。
Objective To get more new information about the antigen epitopes of the hypervariable region 1 (HVR1) within the putative envelope glycoprotein of hepatitis C virus(HCV). Methods We designed and synthesized linear epitope peptides(LP) and multiple antigen peptide(MAP, symmetric & branches) derived from 22 amino acids (position 390-4llaa) of HCV-BJ(HCV fo genotype) HVRI. We compared the binding-reactivity of LP with MAP to native antiHVRI antibody in HCV-infected patients sera. In addition, the HCV genotype was tested to assess the prevalence of antiHVRI by cleaving PCR products with restriction enzyme. Results (l)There was a s;gnificant difference(p < 0.000 l) of mean A450 between anti-MAP (x±s =0.461±0.590) and anti-LP (x± s =0. 145 ± 0. 166), and the immunoreactivity of MAP and LP to the native antibody had a significant association (P < 0.001). (2)The rate of anti-LP and anti-MAP detection in 59 anti-HCV2.0 positive sera was 29% and 58% respectively. (3)The binding of MAP to antibody protein in sera from patients infected with other genotypes besides HCV fo genotype was observed. Conclusion The binding-reactivity of MAP to native antibody was obviously higher than that of LP, and there is a cross--reaction between them.lt was possible to analyse conformational antigen epitopes in the region and develop HCV molecular vaccine by synthesizing MAP corresponding to HCV HVRI sequences.
出处
《中华肝脏病杂志》
CAS
CSCD
2000年第1期48-50,共3页
Chinese Journal of Hepatology
基金
国家自然科学基金资助!39670672
关键词
丙型肝炎病毒
高变区
抗原肽
反应原性
Hepatitis C virus
Hypervariable region
Antigens, synthetic
Vaccine
