摘要
目的:建立一种利用免疫亲合柱纯化细胞的方法。方法:将亲合素交联于聚丙烯酰胺凝胶BioGel上,制成免疫亲合柱;将外周血单个核细胞与生物素化抗CD8单克隆抗体作用后,加到该免疫亲合柱上进行纯化,CD8+细胞即吸附于柱上,洗去其他细胞后,得到纯化的CD8+细胞。结果:纯化后CD8+细胞纯度达56%～7l%,细胞回收率为39%～66%,台盼蓝染色法显示活细胞达98%。结论:利用免疫亲合柱纯化细胞具有纯度高,回收率高,细胞活性好等优点,是一种方便有效的细胞纯化方法。
Objective:To prepare immunoaffinity column for cell separation. Methods: Avidin was attached to Bio Gel and columns packed with Avidin conjugated Bio Gel was prepared. Cells from human peripheral blood were incubated with biotinylated anti CD8 monoclonal antibody ane then applied to the column;Biotin labeled target cells are captured on Avidin coated Bio Gel beads and non target cells were washed off .Bounded target cells were then released by gentle agitation. Results: CD8 +cells were enriched with a purity of 56%～71% and a recovery of 39%～66%;the percent of viable CD8 + cells were proved to be 98% by Trypan Blue Staining. Conclusion: With an high purity and recovery,the immunoaffinity column can be used as an efficient method for cell isolation.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2000年第2期76-78,共3页
Chinese Journal of Immunology
基金
国家自然科学基金!3 9870 72 9
