摘要
目的:应用AFLP技术对吴茱萸的遗传背景进行研究,分析不同产区吴茱萸的遗传特性差异。方法:试剂盒提取吴茱萸幼嫩叶片基因组DNA,构建AFLP实验体系并筛选引物,对来自全国5个省份的46份样品进行遗传背景的研究;用NT-SYS-pc 2.1软件对AFLP扩增结果进行聚类分析。结果:从72对AFLP引物中优选6对用于吴茱萸遗传背景的研究,通过研究发现在相关系数为0.53时,浙江地区的吴茱萸石虎变种样品与其他地区样品分开;石虎变种遗传差异比吴茱萸更显著并呈现出较强的地缘相关性。结论:吴茱萸遗传背景差异明显,AFLP分析方法可有效鉴别不同品种的吴茱萸,并能检测到地区间的差异。
Objective： To study the genetic background of Evodia rutaecarpa by AFLP, and analyze the genetic diversity of E. rutaecarpa from different areas, nethod：E, rutaecarpa genoinic DNA was extracted. The AFLP reaction system was established and AFLP primer pairs were chosen for the analysis. Forty-six individuals of E. rutaecarpa which from five provinces were analyzed by AFLP. The NTSYS-pc 2.1 software was used for cluster analysis. Result： Six out of the original 72 pairs of primers were optimized h）r the study; AFLP analysis revealed the similarity coefficient of 0.53, the samples of E. rutaecarpa var. offocinalis from Zhejiang prov- ince was separated from other accessions; E. rutaecarpa var. officinalis also showed more pronounced genetic variation than the E. rutaecarpa, and strong geo-related relevance. Conclusion： Variance of genetic background of E. rutaecarpa are large, AFLP analysis method can obviously identify＂ different varieties of E. rutaecarpa, and can detect the genetic characteristics of inter-regional differences.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第3期278-282,共5页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(30973877)
国家“十一五”科技支撑计划项目(2006BAI09B02)
湖南省科学技术厅科技项目(2008SK3087)
国家中医药管理局“中药植物学”重点学科项目(国中医药发[2009]30号)
湖南省高校“中药新药创制与资源综合利用”科技创新团队项目(湘教通[2010]212号)
湖南省“中药学”重点学科项目
关键词
吴茱萸
AFLP
道地药材
聚丙烯酰胺凝胶电泳
银染
Evodia rutaecarpa
AFLP
geo-authentic cnlde drugs
polyacrylamide gel electrophoresis
silver staining
