摘要
利用荧光检测技术以外消旋帕罗西汀中间体为底物,乙烯乙酸酯为酰基供体,建立脂肪酶酯化活性的高通量筛选模型,以筛选获得具有较高酯化活性的微生物.实验中荧光激发波长为485nm,荧光吸收波长为538nm,建立了筛选模型的工作曲线Y=10 685X+176.15,具有良好的线性关系,相关系数R2=0.997 2.
A fluorescence-based high throughput model is established for screening of lipase-producing microorganisms by taking rac-paroxetine intermediate as substrate and taking vinyl acetate as acyl donor,respectively.In the experiment,fluorescent excitation wavelengths is 485 nm and fluorescent emission wavelengths is 538 nm.The work curve of screening model is Y=10 685X＋176.15,which linearly correlated with the correlation and coefficient of 0.997 2.
出处
《浙江工业大学学报》
CAS
2012年第1期39-41,共3页
Journal of Zhejiang University of Technology
基金
浙江工业大学校级科学研究基金项目(20090110)的资助
