摘要
目的探讨在影响妊娠期糖尿病大鼠胎膜通透性方面的可能机制。方法选取造模成功的妊娠期糖尿病大鼠(模型组)和正常妊娠大鼠(对照组)各30只,在妊娠19d时于无菌条件下留取大小1.0cm×1.0cm胎膜2块,采用RT-PCR反应检测胎膜中AQP8mRNA的表达。结果 2组大鼠胎膜上均有AQP8mRNA的表达,且AQP8mRNA在模型组大鼠胎膜上的表达明显低于对照组(P<0.05)。结论水通道蛋白8mRNA在孕足月大鼠胎膜有表达,其在妊娠期糖尿病大鼠胎膜中的表达降低可能参与了羊水量异常的调控。
Objectives To investigate the possible mechanism of permeability changes in fetal membrane of gestational diabetes mellitus （GDM） rats by detecting the aquaporin 8 （AQP8） gene＇s expression in the fetal membrane by using the reverse transcription - polymerase chain reaction （RT-PCR） ＇technique. Methods The 30 gestational diabetic rats （model group） and 30 normal full-term pregnancy rats （control group） were enrolled in the study. At 19 days after pregnancy,two pieces of fetal membranes with size about 1.0 cm x 1.0 cm were taken out under sterile condition, and the expression of AQP8 mRNA in fetal membrane was detected by RT-PCR. Results The both groups showed the expression of AQP8 mRNA in fetal membrane of rats, furthermore, the expression of AQP8 mRNA in fetal membrane of the rats of model group was significantly lower than that of rats in control group （ P 〈 0.05）~ Conclusion The aquaporins 8 mRNA expresses in the fetal membrane of full-term pregnancy rats,and the decrease of expression in the fetal membrane of gestational diabetic mellitus rats may be involved in the regulation of the abnormality of amniotic fluid amount.
出处
《河北医药》
CAS
2012年第3期339-340,共2页
Hebei Medical Journal
关键词
水通道蛋白
胎膜
妊娠
糖尿病
aquaporins
fetal membrane
pregnancy
gestational diabetes
