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骨髓和脂肪来源的间充质干细胞免疫调节作用的比较研究

The research on the immuno-modulatory abilities of bone marrow-derived and fat-derived MSCs
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摘要 本研究中我们分离骨髓和脂肪的间充质干细胞,分别检测它们对T细胞周期、活化、抑制和增殖的作用情况,研究脂肪来源(adipose-derived mesenchemal stem cell,AMSC)和骨髓来源(bone marrow derived mesenchemal stem cell,BMSC)间充质干细胞的免疫学特征,比较它们是否存在免疫功能的差异,结果显示AMSC和BMSC均具有抑制T细胞增殖的能力,在有丝分裂原刺激和MLR的T细胞增殖中均具有剂量依赖性的,在1∶2时有极强的抑制作用,但是在1∶100时这种作用基本消失,在共培养时AMSC和BMSC都可使更多的T细胞被抑制在G0/G1期,同时也可以抑制T细胞的早期活化,但是上述作用AMSC均较BMSC弱,但是AMSC并不具有抑制T细胞凋亡的作用,而两者在Th0向Th1或Th2分化中所起的作用是基本相似的,主要抑制Th0向Th1细胞(IL-2和IFN-γ生成细胞)的分化,而对向Th2细胞(IL-4和IL-10生成细胞)分化没有明显的影响。所以,AMSC和BMSC具有类似的免疫调节作用,因AMSC取材方便而成为更佳的材料来源。 To investigate that whether the immuno-modulatory capacity is intact in bone marrow-and fat derived-mesenchymal stem cells（MSCs）,MSCs from the CML patients and the normal donors were isolated and the differences between the two groupe in the capability of T lymphocyte activation,proliferation and suppression were examined.The results showed that the capacity of suppressing T cell proliferation and activation is weakened.We propose that MSCs from pathological environment might be abnormal and should not be used for autologous transplantation.
作者 朱希山 台卫平 施薇 安广宇
机构地区 首都医科大学附属北京世纪坛医院〈北京大学第九临床医学院〉肿瘤内科
出处 《现代免疫学》 CAS CSCD 北大核心 2012年第1期56-63,共8页 Current Immunology
关键词 脂肪来源间充质干细胞 骨髓来源间充质干细胞 免疫调节 mesenchymal stem cell bone marrow derived adipose derived immuno-modulatory
分类号 R392.12 [医药卫生—免疫学]
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参考文献35

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现代免疫学
2012年 第1期

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