摘要
[目的]分别研究鹿蹄草等4种中草药提取液对桃褐腐菌的抑制效果。[方法]采用3次浸渍提取法对中草药进行提取,通过菌丝生长速率法、单位面积产孢量计算法和凹玻片悬低法进行抑菌活性测定。[结果]在离体条件下鹿蹄草、丁香、甘草、葛根等4种中草药提取液对桃褐腐病菌营养生长、分生孢子的产生与萌发都有不同程度的抑制作用,且对菌丝细胞也有一定程度的破坏作用。鹿蹄草提取液对桃褐腐病菌的生长抑制率均达到了80%以上,丁香水蒸气提取液抑菌效率达90%以上。[结论]鹿蹄草提取液和丁香水蒸气提取液的抑菌效果较为明显,说明鹿蹄草提取液和丁香水蒸气提取液中含有抑菌成分。
[Objective]The paper was to study the inhibitory effects of four Chinese herbal extracts containing Pyrola rotundifolia against Monilinia fructicola.[Method]Three-time-impregnation method was used to extract Chinese herbs.The antifungal activity was determined by methods of mycelial growth rate,sporulation per unit area and germination rate on slide.[Result]Pyrola,clove,licorice and kudzu root extracts in vitro had different degrees of inhibition effects on vegetative growth,conidia production and germination of M.fructicola,which also had certain degree of destruction effect on mycelial cells.Growth inhibition rate of Pyrola extract against M.fructicola reached 80%,and that of water vapor extract of clove was more than 90%.It also had significant destruction effect on mycelial cells,and could inhibit the production and germination of conidia.[Conclusion]Inhibition effects of Pyrola extract and water vapor extract of clove were obvious,indicating that they had antifungal composition.
出处
《安徽农业科学》
CAS
北大核心
2011年第23期14175-14177,共3页
Journal of Anhui Agricultural Sciences
基金
北京市教育委员会"专业建设--食品质量与安全本科专业实践教学体系建设"(PXM2011-104207-000059)
北京市教育委员会"酵母菌拮抗菌机制研究"(5075232071)
关键词
中草药
桃褐腐菌
抑菌
机理
Chinese herbal medicine
Monilinia fructicola
Antifungal
Mechanism
