摘要
[目的]从鲜牛奶中分离植物乳杆菌,为牛奶中植物乳杆菌的开发利用提供依据。[方法]将鲜牛奶稀释,进行涂布转接,再通过活菌培养、形态特征观察、生理生化试验,结合16S rDNA序列分析进行鉴定。[结果]从鲜牛奶中分离得到1株细菌(D14),菌体形态为杆状,单个或链状排列,无芽孢,菌落凸起,边缘光滑细腻,呈白色,直径0.4～2.2 mm,有酸味。再结合各生理生化试验和16S rDNA序列分析,将其鉴定为植物乳杆菌(Lactobacillus plantarum)。[结论]从鲜牛奶中分离得到的植物乳杆菌D14来源安全,有望应用于食品和饲料发酵,具有一定的应用前景。
[Objective]The study aimed to isolate Lactobacillus plantarum from the fresh milk and provide the basis for the development and utilization of plant lactobacillus.[Method]The fresh milk was diluted to conduct the coating connecting and then through living bacterium culture,morphology observation,physiological and biochemical tests,combining 16S rDNA sequence analysis,the bacterium was identified.[Result]A strain of bacterium named as D14 was isolated from the fresh milk.The morphology of the bacteria was rod-shaped,individual or chain-shaped arrangement,no spores,colonies protuberant,edge smooth and fine,white,0.4-2.2 mm in diameter,with sour taste.Combined with the physiological and biochemical tests and 16S rDNA sequence analysis,the bacterium was identified as L.plantarum.[Conclusion]The L.plantarum D14 isolated from the fresh milk was safe in the source,so it is expected to be used for the food and feed fermentation,which had certain application prospect.
出处
《安徽农业科学》
CAS
北大核心
2011年第23期14113-14114,14122,共3页
Journal of Anhui Agricultural Sciences
基金
云南省自然科学基金重点项目(2009CC005)
