摘要
目的探讨长波紫外线(UVA)照射对大鼠机体氧化损伤的影响。方法采用长波紫外线为照射源,对大鼠进行照射,检测各组血清及肝脏匀浆中氧化物歧化酶(SOD)和谷胱甘肽(GSH)活性,丙二醛(MDA)含量及抑制羟自由基的能力。结果经981.71 J/cm2 UVA照射后,模型组血清中SOD、GSH活性明显低于正常组(P<0.05);羟自由基、MDA含量显著高于正常组(P<0.05)。肝组织匀浆中,模型组SOD活性、抑制羟自由基能力明显低于正常组(P<0.05);但MDA与GSH与正常组无统计学意义(P>0.05)。结论 UVA可对大鼠造成氧化损伤。
Objective To elucidate the effect of long-wave ultraviolet（UVA） irradiation on the rats. Methods The rats were exposed to the ultraviolet for 13 weeks. And after that, the activity of SOD, GSH, MDA content and the ability of inhibiting the hydroxyl radical in serum and liver homogenate were detected. Results After 981.71 J/CM2 UVA irradiation, the SOD and GSH activity in serum in the model group was significantly lower than those in the control group（P 〈 0. 05）, hydroxyl radicals and MDA levels were obviously higher than those in the control group （P 〈 0.05 ）. SOD activity and inhibition of hydroxyl radicals of the model group in liver tissue was much lower than those in the control group （ P 〈 0.05 ） ; but there were no significant difference in MDA and GSH between the model group and the control group （P 〉 0.05 ） .Conclusion UVA can cause oxidative damage in rats.
出处
《吉林医药学院学报》
2011年第5期257-259,共3页
Journal of Jilin Medical University
基金
吉林医药学院大学生科研基金(200903)
