摘要
目的】研究尿激酶型纤溶酶原激活物UPA及其特异受体UPAR和抑制物PAI1,PAI2与肺非小细胞癌(NSCLC)转移的关系。【方法】用免疫组化LSAB法检测UPA,UPAR,PAI1和PAI2在66例肺NSCLC中蛋白水平表达。【结果】肺腺癌,鳞癌有淋巴结转移组UPA,PAI1阳性率及表达强度均比无转移组高,P值均<005。肺大细胞癌有淋巴结转移组UPA,UPAR阳性率比无转移组高,P=0001和P=003。对4种因素UPA,UPAR,PAI1,PAI2与肺癌淋巴结转移关系行logistic回归分析,UPA,PAI1,PAI2三个因素引入回归方程,P=0001,P=0031和P=0043。UPA,PAI1回归系数均为正值,相对危险度(RR)为4170和2177,而PAI2回归系数为负值,RR=0504。【结论】UPA、PAI1与肺腺癌、鳞癌转移密切相关,UPA,UPAR与肺大细胞癌转移相关,UPA,PAI1是肺癌淋巴结转移促进因子,而PAI2是其抑制因子。
Objective To study the role of UPA,UPAR,PAI 1 and PAI 2 in metastasis of non small cell lung cancer(NSCLC). Methods The expression at protein level for UPA,UPAR,PAI 1 and PAI 2 were examined in 66 cases of NSCLC. Results The positive rate as well as quantitative assay of immune staining for UPA and PAI 1were significantly higher (P<0.05)in cases with lymph node involvement (LN+) than those without lymph node involvement(LN ) in adenocarcinoma(AC) and squamous cell carcinoma(SCC). In large cell lung cancer(LCLC),the positive rate for UPA,UPAR was significantly higher in LN+ cases than those in LN cases(P=0.001 and P=0.03). The relationship between 4 factors and lymph node involvement was analyzed by multivariate backward logistic regression model. The result showed that UPA,PAI 1 and PAI 2 entered the regression model (P=0.001,P=0.031, and P=0.043 respectively).Regression coefficient of UPA and PAI 1 was positive,RR=4.170 and RR=2.177,while that of PAI 2 was negative ,RR=0.504. Conclusions UPA and PAI 1 are associated with metastasis in adenocarcinoma and squamous cell carcinoma. While UPA and UPAR are associated with metastasis in large cell lung cancer.UPA,PAI 1 may contribute to tumor metastasis, whereas PAI 2 may prevent tumor metastasis.
出处
《中山医科大学学报》
CSCD
2000年第1期14-16,20,共4页
Academic Journal of Sun Yat-sen University of Medical Sciences
基金
国家自然科学基金!资助课题 (39570 2 940 0 )
关键词
尿激酶
肺肿瘤
肿瘤转移
免疫组织化学
urokinase
lung neoplasms
neoplasm metastasis
immunohistochemistry
