摘要
1963年美国化学家M.C.Wani和M.E.Wall首次从一种生长在美国西部森林中称为太平洋紫杉（Pacific yew）的树皮和木材中分离到了紫杉醇粗提物。筛选实验发现,紫杉醇粗提物对离体培养的鼠肿瘤细胞有很高的活性。
Established method by reverse phase HPLC and electrospray ionization mass spectrometry（HPLC-ESI-MS） analysis and hazelnut extract trace of taxol.Through the research,seven mass spectrum characteristics of taxol compounds were contested,and the taxanes database was initially established.It was found that the paciltaxel contents were 23.65 μg·mL-1 and 4.09 μg·mL-1 in leaves and stems respectively.The paciltaxel contents in different parts of hazelnut was slightly different,which were 0.61,0.23 and 0.35 μg·mL-1 in hazel branch,leaf and leather.With these data it can quickly identify the known paclitaxel compounds in hazelnut extract,and can perform the unknown structure analysis to certain degrees.This analysis method is confirmed sensitive,reliable and fast.
出处
《林业科学研究》
CSCD
北大核心
2011年第6期779-783,共5页
Forest Research
基金
南京中医药大学亚热带林业研究所所长基金(RISF6921)
浙江省省院合作林业科技项目(2007SY10)
