摘要
以草酸青霉(Penicillium oxalicum)D1为出发菌株,经过紫外线、亚硝酸以及紫外与亚硝酸复合诱变处理,选育出1株高产纤维素酶突变株NU-H,与出发菌株相比,CMCase酶活提高69.8%,滤纸酶活提高75.28%,另外木聚糖酶活,还原糖得率也有显著地提高。通过单因子及正交实验,研究了突变株纤维素酶对稻草粉水解的最适宜条件。结果表明,该菌株酶解糖化的最佳条件为:温度50℃,时间28 h,纤维素酶浓度为60%,底物浓度3%,pH 4.8,还原糖得率为23.25%。
A strain（NU-H） with high cellulase production was selected from Penicillium oxalicum D1 by ultraviolet mutation,nitrous acid mutation and the compound mutation of ultraviolet and nitrous acid.As compared with the original strain,the carboxymethyl cellulase（CMCase） and filter paper enzyme activities of NU-H increased by 69.08% and 75.28%,respectively.A significant increase of xylanase activity and reducing sugar yield was also observed.The single factor experiments and the resulting orthogonal experiments were designed to deter mine the optimal enzyme hydrolysis conditions of straw using the cellulase produced by NU-H.Results showed that the optimal enzyme hydrolysis conditions were as follows： temperature of 50℃,time of 28 h,enzyme concentration of 60%,substrate concentration of 3%,pH of 4.8.Under these conditions,the yield of reducing sugar was up to 23.25%.
出处
《环境工程学报》
CAS
CSCD
北大核心
2012年第2期658-664,共7页
Chinese Journal of Environmental Engineering
基金
国家自然科学基金资助项目(30901010)
国际科学基金IFS资助项目(F/4589-1)
湖南省自然科学基金资助项目(08JJ6020)
关键词
草酸青霉
纤维素酶
诱变
酶水解
Penicillium oxalicum
cellulase
mutation
enzyme hydrolysis
