摘要
从某炼油厂受石油污染的土壤中分离、筛选得到一株高效烷烃降解菌株C18,经形态观察、生理生化实验和16S rDNA序列分析确定其为变形假单胞菌。采用静态摇瓶实验,研究了菌株C18对正十六烷的降解条件和动力学特性,并应用于柴油的降解。菌株降解正十六烷的最适宜条件为温度30～37℃、初始pH=7、盐质量分数1%,且在pH为6～9和高盐含量(NaCl质量分数5%)条件下也有良好的降解能力。动力学研究显示,在底物浓度为10～300mg/L时,获得的米氏方程中,米氏方程常数Km为15.22mg/L,最大反应速率vmax为11.22mg/(L.h)。在60h内,优势菌C18不仅能将浓度为3 000mg/L的柴油中饱和中长链烷烃高效降解,而且对其中的环烷烃和芳烃也具有良好的降解能力。
A high effective alkane-degradation bacterial strain C18 was isolated from oil-contaminated soil of a refinery, and it was identified as Pseudomonas plecoglossicida based on its morphological feature,biochemical ＆ physiological characteristics and partial 16S rDNA sequence analysis. The ability of degrading n-hexadecane/diesel and growth kinetics of said strain were studied by shaking flask tests. It was found that the optimum conditions for the growth of strain C18 were under 30--37℃ ,system pH= 7 and containing 1% NaCl. Even at pH = 6--9 and system containing 5% NaCl,its ability to degrade n-hexadecane was still high. The growth kinetics research showed that when the concentration of n-hexadecane was 10 300 mg/L under optimum conditions,Michaelis equation could be acquired by calcula- tion with Kr, being 15. 22 mg/L and Vmax being 11.22 mg/（L·h）. Under a diesel concentration of 3 000 mg/L, dominant bacterial strain C18 not only could degrade n-alkanes in diesel fuel effectively, but also could well degrade the cyclanes and aromatic hydrocarbons in diesel fuel within 60 h.
出处
《石油炼制与化工》
CAS
CSCD
北大核心
2012年第2期28-32,共5页
Petroleum Processing and Petrochemicals
基金
湖北省教育厅产学研重点项目(CXY2009B002)
武汉市供需对接项目(200951624497)
关键词
正十六烷
优势菌
降解特性
降解动力学
n-hexadecane
dominant bacteria
degradation performance
degradation kinetics
