摘要
将来自川东、南稻区代表性的38个县(市)的水稻纹枯病标样29 6份,按不同的品种、海拔、土质、前作和症状归类,选代表性的标样分离得到10 8个丝核菌菌株。按HCL-Giem sa染色程序和菌丝融合测定法,将108个菌株分为3个菌系:Rhizoctonia solani AG-1和AG-4,以及双核丝核菌的AG-Bb。其比例分别为97%、1%和2%。经致病性测定表明,该各菌系对水稻的致病性有显著差异:R.solani AG-1的大多数菌株最强,双核丝核菌AG-Bb最弱,R.solani AG一4居中。对上述各菌系的培养性状、非特异性酯酶和过氧化物酶同工酶谱进行比较研究发现,不同菌系间在上述诸方面均存在明显差异,而同菌系不同菌株间却具一致性。由此说明,按菌丝融合与否区分丝核菌种群较之现行的其它分类法更能反映其遗传本质和亲缘关系。
108 isolates of Rhizoctonia spp.were isolated from diseased tissues of sheath blight of rice in 38 counties in the east and south of Sichuan province.They were divided into two series of Rhizoctonia:multinucleate and binucleate series.According to the hyphal fusion with Ogoshi's anastomosis groups (AGs) of Rhizoctonia,the multinucleate and binucleate series belong to R.solani AG-1(97%) and AG-4(1%),AG-Bb(2%),respectivily.The results of pathogenicity test on rice indicated that most isolates of AG-1 had the strongest pathogenicity to rice,the isolates of AG-Bb had the least pathogenicity to rice,and the isolates of AG-4 had mid-pathogenicity. Comparisons in culture characteristics,non-specific esterase zymojrams,and peroxidase zymograms of the above three groups of Rhizoctonia were made in 1987—1988.The results showed that they were different among the various AGs but similar among the various isolates in the same AG.It seems that the above zymograms reaction may be used as a criterion in the classification of Rhizoctonia.
出处
《真菌学报》
CSCD
北大核心
1990年第1期41-49,共9页
关键词
水稻
纹枯病
菌系
丝核菌属
Rice
Sheath Blight
Strain
Rhizoctonia
