摘要
目的探讨急性白血病（AL）p27基因mRNA的表达及临床意义。方法采用反转录聚合酶链反应（RT-PCR）检测65例初治AL和41例造血系统非恶性肿瘤患者（对照组）骨髓单个核细胞中p27基因mRNA表达水平，分析p27mRNA表达与AL患者的年龄、性别、外周血白细胞计数和髓外浸润临床特征的相关性。结果AL中p27基因mRNA表达的阳性率为36．9％（24／65），显著低于对照组的87．8％（36／41），差异有统计学意义（P〈0．05），急性髓系白血病（AML）组和急性淋巴细胞白血病（ALL）组分别为34．2％（13／38）和37．5％（9／24），均低于对照组，差异有统计学意义（均P〈0．05），但AML组和ALL组比较，差异无统计学意义（P〉0．05）。p27mRNA表达年龄≥14岁组（52例）和〈14岁组（13例）比较，差异无统计学意义（P〉0．05）；男性组（34例）和女性组（31例）比较，差异无统计学意义（P〉0．05）；外周血白细胞计数≥20X10。／L组（31例）和〈20×109／L组（34例）比较，p27mRNA表达与白细胞计数呈负相关（r=-0．284，P〈0．05）；有髓外浸润组（37例）和无髓外浸润组（28例）比较，p27mRNA表达与髓外浸润呈负相关（r=-0．300，P〈0．05）。结论AL存在p27基因mRNA的表达缺失，缺失同时存在于AML和ALL中，缺失与患者外周血白细胞计数、髓外浸润呈负相关，这可能是AL发生、发展的机制之一。
Objective To explore the p27 gene expression and clinical significance in acute leukemia （AL）. Methods RT-PCR was used for testing the mRNA levels of p27 gene in bone marrow mononuclear cell of 65 cases of newly diagnosed AL patients and 41 controls. The correlation between the p27 gene expression positive rate and age, sex, peripheral blood white blood cell count and extramedullary infiltration were analyzed simultaneously in AL patients. Results The positive rate of p27 gene expression （36.9 %, 24/65） was significantly lower in AL group than that in the control group （87.8 %, 36/41） （P 〈0.05）, and which was lower than the control group （P 〈0.05） in the AML group （34.2 %, 13/38） and the ALL group （37.5 %, 9/24）. However, comparing AML group with ALL group, the difference was not statistically significant （P 〉0.05）. At the same time, compared the p27 gene expression positive rate of ≥ 14 years old group （52 cases） and 〈14 years old group （13 cases）, and also compared in male group （34 cases） and in female group （31 cases）, there was no statistically difference （P 〉0.05）; Compared the p27 gene expression positive rate of peripheral blood white blood cell count ≥20×10^9/L group （31 cases） and 〈20×10^9/L group （34 cases）, also compared with in extramedullary infiltration group （37 cases） and in no extramedullary infiltration group （28 cases）, the relationship of p27 gene expression positive rate with peripheral blood white blood cell count as well as extramedullary infiltration was a negative correlation （r = -0.284, P 〈 0.05, r = -0.300, P 〈 0.05）. Conclusion There is p27 mRNA expression deletion in AL patients, and the deletion is present in the AML and ALL at the same time. p27 mRNA expression is negatively correlated with peripheral blood white blood cell count or extramedullary infiltration, which might be one of the mechanisms of occurrence and development of AL.
出处
《白血病．淋巴瘤》
CAS
2011年第12期716-718,共3页
Journal of Leukemia & Lymphoma
基金
昆明医学院研究生创新基金（KM2008L17）
