摘要
目的:观察水蛭素对肝癌H22荷瘤小鼠的抑瘤作用。方法:小鼠右前腋窝下皮下种瘤造成实体瘤模型,将40只小鼠随机抽样分为4组,水蛭素高剂量和水蛭素低剂量组、氟尿嘧啶组及模型对照组,每组10只。治疗期间记录各组小鼠的体重变化并观察小鼠生存状态,用药14 d后取瘤称重,计算抑瘤率,并计算肝/体比、肾/体比、脾脏指数以及用药前后的体重变化等指标。结果:水蛭素高、低剂量组对小鼠H22肝癌细胞均有明显的抑制作用,抑瘤率分别为37.63%、23.71%,与模型对照组比较,差异有统计学意义(P<0.01、P<0.05),水蛭素高、低剂量组与氟尿嘧啶组比较,肝/体比、肾/体比、脾脏指数均有显著性差异,有统计学意义(P<0.05),经治疗后水蛭素各组小鼠与模型对照组及治疗前比较体重变化不明显(P>0.05)。结论:水蛭素对小鼠H22肝癌细胞有明显的抑制作用,同时对小鼠重要器官可能具有一定的保护作用。
OBJECTIVE： To evaluate the antitumor effect of hirudin on H22 tumor cells in mice.METHODS： 40 mice were injected with H22 hepatoma cells from their right axillae to establish solid tumor model before being randomly divided into 4 groups of 10 each： high dose hirudin group,and low dose hirudin group,5-FU group and model control group.The body weight and survival of mice in all groups were recorded.After exposing to medications for 14 days,the tumors were taken out and weighted with tumor inhibition rate,liver/body ratio,kidney/body ratio and spleen indices computed and body weight before and after medication recorded.RESULTS： Hirudin at either high dose or low dose significantly inhibited H22 tumor cells in mice,with tumor inhibition rates of 37.63% and 23.71%,respectively,showing significant differences compared to model control group（P0.01,P0.05）.The high dose hirudin group and low dose hirudin group differed greatly from 5-FU group with regard to liver/body ratio,kidney/body ratio and spleen indices（P0.05）.Weight change of the hirudin groups（high dose or low dose） was not significant as compared with before treatment or compared with the model control group（P0.05）.CONCLUSION： Hirudin has significant antitumor effect on H22 tumor cells and protective effect on vital organs in mice.
出处
《中国医院用药评价与分析》
2011年第12期1105-1107,共3页
Evaluation and Analysis of Drug-use in Hospitals of China
