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白念珠菌pH应答转录因子Rim101p在大肠杆菌中的表达 被引量：1

The Expression of Candida albcians Transcirptial Factor Rim101p in E. coli
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摘要 Rim101蛋白是白念珠菌中pH应答反应的重要转录因子.为进一步研究Rim101p的功能,采用PCR方法获得RIM101基因序列,并将其克隆入表达载体pET-21a,转化大肠杆菌表达菌BL21,通过摸索诱导条件使Rim101p在大肠杆菌中成功表达.EMSA实验结果进一步验证了重组Rim101p的活性. In Candida albicans,Rim101p is an important transcripital factor to pH response.For further research on the function of Rim101p,Rim101 gene was cloned,expression voctor pET-21a-Rim101 was constructed and Rim101p express in E.coli was made successfully.EMSA results suggest that Rim101p expressed in E.coli had the ability to bind GCCAAGAA element.
作者 桂磊 郑雯 邢来君 李明春
机构地区 南开大学微生物学系分子微生物学与技术教育部重点实验室
出处 《南开大学学报（自然科学版）》 CAS CSCD 北大核心 2011年第5期96-100,共5页 Acta Scientiarum Naturalium Universitatis Nankaiensis
基金 国家自然科学基金(31070126 81171541) 天津市应用基础及前沿技术研究计划(10JCYBJC09700)
关键词 白念珠菌 Rim101p EMSA Candida albicans Rim101p electrophoretic mobility shift assay（EMSA）
分类号 Q78 [生物学—分子生物学]
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参考文献11

  • 1Davis D A. Adaptation to environmental pH in Candida albican and its relation to pathogenesis [J]. Curr Genet, 2003, 44(1): 1--7.
  • 2Chiaffin W L, Lepez-Ribot J L, Casanova M, et al. Cell wall and secreted proteins of Candida albicans: identifica- tion, function, and expression[J]. Mierobiol Mol boil Rev, 1998, 62(1): 130--180.
  • 3Davis D A, Wilson R B, Mitchell A P. RIMlOl-dependent and-independent pathways govern pH response in Candi- da albicans[J]. Mol Cell Biol, 2000, 20(3): 971--978.
  • 4Li M C, Martin S J, Bruno V M, et al . Candida albicans Riml3p, a preotease required for Riml01p processing at acidic and alkaline pHs[J]. Eukaryotic cell, 2004, 3(3):741--751.
  • 5Davis D A, Edwards J E, Mitchell A P, et al. Candida albicans RIM101 pH response pathway is required for host- pathogen interactions[J]. Infect Immun, 2000, 68(10): 5 953--5 959.
  • 6Ramon A M, Fonzi W A. Diverged binding specificity of Rim101p, the Candida albicans ortholog of PacC[J]. Eu- karyotic Cell, 2003, 2(4): 718-728.
  • 7Yong-Un B, Samuel J M, Dana A D. Evidence for novel pH-dependent regulation of Candida albicans Rim101, a di- rect transcriptional repressor of the cell wall fl-glycosidase PHR2[J]. Eukaryotic Cell, 2006, 5(9).. 1 550--1 559.
  • 8李艳琴,孙永艳.可用于基因检测的啤酒发酵液DNA提取方法研究[J].食品科学,2007,28(3):191-194. 被引量：6
  • 9Sascha T, Marianne K, Hyunsook P, et al. In vivo and ex vivo comparative transcriptional profiling of invasive and non-invasive Candida albicans isolates identifies genes associated with tissue invasion[J]. Molecular Microbiology, 2007, 63(6): 1 606-1 628.
  • 10Eric S B, Samuel J M, Li M C, et al. Transcriptional profiling in Candida albicans reveals new adaptive responses to extracellular pH and functions for Rim101p[[J]. Molecular Microbiology, 2004, 54(5): 1 335--1 351.

二级参考文献36

  • 1朱衡,瞿峰,朱立煌.利用氯化苄提取适于分子生物学分析的真菌 DNA[J].真菌学报,1994,13(1):34-40. 被引量：307
  • 2BARSZCZEWSKI W,ROBAK M.Differentiation of contaminating yeasts in brewery by PCR-based techniques[J].Food Microbiology,2004,21,227-231.
  • 3SATOKARI R,JUVONEN R,MALLISON K,et al.Detection of beer spoilage bacteria Megasphaera and Pectinatus by PCR and colorimetric microplate hybridization[J].International Journal of Food Microbiology,1998,45:119-127.
  • 4TOMPKINS T A,STEWART R,SAVARD L,et al.RAPD-PCR characterization of brewery yeast and beer spoilage bacteria[J].J Am Soc Brew Chem,1996,54:91-96.
  • 5SAKAMOTO K,KONINGS W M.Beer spoilage bacteria and hop resistance[J].International Journal of Food Microbiology,2003,89:105-124.
  • 6WILLIAM J G K,KUBELIK A R,LIVAK K J,et al.DNA polymorphisms amplified by primers are useful as genetic markers[J].Nucl Acids Res,1990,18:6531-6535.
  • 7ANDRIGHETTO C,PSOMAS E,TZANETA Is N,et al.RAPD for the idenification of yeasts isolated from dairy products[J].Lett Appl Microbiol,2000,30:5-9.
  • 8Davis DA.Adaptation to environmental pH in Candida albican and its relation to pathogenesis.Curr Genet,2003,44:1-7.
  • 9Espeso EA,Penalva MA.Three binding sites for the Aspergillus nidulans PacC zinc-finger transcription factor are necessary and sufficient for regulation by ambient pH of the isopenicillin N synthase gene promoter.J Biol Chem,1996,271:28825-28830.
  • 10Suarez T,Penalva MA.Characterization of a Penicillium chrysogenum gene encoding a PacC transcription factor and its binding sites in the divergent pcbAB-pcbC promoter of the penicillin biosynthetic cluster.Mol Microbiol,1996,20:529-540.

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南开大学学报（自然科学版）
2011年 第5期

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