摘要
目的通过观察游泳训练对糖尿病大鼠胰腺组织抗氧化作用及超微结构的影响，探讨有氧运动对糖尿病大鼠胰腺线粒体保护作用的机制。方法健康sD大鼠45只，按照随机数字表法，选取10只作为正常对照组；另35只采用链脲佐菌素（STZ）注射诱导1型糖尿病动物模型，剔除造模未达标大鼠5只，糖尿病大鼠模型造模成功率为85．7％。从造模成功的30只中，选取能够屏蔽胰岛素抵抗的1型糖尿病大鼠20只作为糖尿病组，并随机分为糖尿病模型组和糖尿病运动组，每组各10只。正常对照组和糖尿病模型组不予运动，定时将鼠笼置于运动组大鼠运动时的桶旁，摒除环境噪音的影响；糖尿病运动组进行中等强度游泳训练，每次60min，每周5d，共8周。实验8周后分别检测正常对照组、糖尿病模型组及糖尿病运动组大鼠的胰腺线粒体中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）及一氧化氮合酶（NOS）活力；测定胰腺线粒体丙二醛（MDA）及一氧化氮（NO）含量；并通过电镜观察3组大鼠胰腺组织超微结构的变化。结果糖尿病运动组大鼠经过8周规律游泳训练后，胰腺线粒体SOD、GPx活力较糖尿病模型组有显著升高（P〈0．05），而NOS活力、MDA及NO含量明显降低（P〈0．05）；糖尿病运动组大鼠胰腺组织超微结构较糖尿病模型组得到明显改善，并与正常对照组形态近似。结论有氧运动对糖尿病大鼠胰腺有保护作用，能增强糖尿病大鼠胰腺组织抗氧化清除自由基的能力，明显改善胰腺组织的超微结构。
Objective To investigate the effects of aerobic exercise training on oxidative stress and ultra- structure of pancreas tissue in diabetic rats and the mechanism of protective effects of aerobic exercise training on pancreatic mitochondria in diabetic rats. Methods Thirty Sprague-Dawley rats were randomly divided into three groups： a normal control group, a diabetic model group and an exercise diabetic group,with 10 rats in each group. Type 2 diabetic rat models were established by intra-abdominal injection of streptozotocin （STZ）. The success rate of establishing diabetic rat models was 85.7% . The normal control group and diabetic model group were not given exercise training. Exercise group was trained with 8 weeks of swimming of medium strength, eg. 60 min/time, 5 d/week. After training for 8 weeks, the activities of superoxide dismutase （SOD） , glutathion peroxidase （GPX） , nitric oxide syntbase （NOS） and the contents of malondialdehyde （MDA） and nitric oxide （NO） in pan- creatic mitochondria were observed respectively. The uhrastructures of pancreas tissue in rats were observed under electron microscope. Results After swimming training for 8 weeks, the activities of SOD and GPx in pancreatic mitochondria in exercise diabetic group elevated more obviously than those in diabetic model group （ P 〈 0.05） , while the activity of NOS and the contents of MDA and NO reduced obviously （P 〈 0.05 ）. Compared with diabetic model group, the uhrastructures of pancreas tissue in exercise diabetic group improved, and the histomorphology was similar to normal control group. Conclusions Aerobic exercise training can provide protective effects on pancreas in diabetic rats, and can enhance antioxidation ability to eliminate free radicals, improve the ultrastructures of pancreas tissue.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2012年第1期12-16,共5页
Chinese Journal of Physical Medicine and Rehabilitation
关键词
有氧运动
糖尿病
胰腺
氧化应激
超微结构
Aerobic exercise
Diabetes mellitus
Pancreas
Oxidative stress
Ultrastructure
