摘要
背景:各种原因造成的骨折和骨缺损导致的骨不连一直是临床骨科修复的一大难题,相关产品质量的检验需要标准的骨缺损模型,然而骨缺损的临界长度至今仍无定论。目的:建立兔桡骨骨缺损模型,以确定兔桡骨临界骨缺损长度。方法:将18只雄性新西兰大白兔,随机分为6组,在双侧桡骨中段分别做12,13,14,15,16,17mm的缺损,伤口缝合包扎但不固定。结果与结论:大体标本和放射学观察显示,3个月内12,13,14,15,16mm组的缺损均有修复完整的情况,但17mm组无一例修复。组织学结果显示12,13,14,15,16mm组缺损修复区有骨小梁与骨基质的形成、骨再生和再血管化、髓腔不同程度再通以及成骨细胞,17mm组可见成骨细胞,破骨细胞,但未见骨再血管化和髓腔再通。故兔桡骨临界骨缺损长度为17mm。
BACKGROUND： Nonunion caused by various reasons of fractures and bone defects has been a major problem of clinical orthopedic rehabilitation. With the rapid development of tissue engineering, product quality testing needs standard bone defect model. However, the length of bone critical-size defects has not been ascertained. OBJECTIVE： To establish rabbit radial bone defect models and to determine the length of rabbit radial bone critical-size defects METHODS： Eighteen male New Zealand white rabbits （2.0 2.5 kg） were randomly assigned into 6 groups and then 12, 13, 14, 11, 16 and 17 mm segmental defects were created in the middle part of radius on both sides, respectively. The incisions were sutured and bandaged, but not fixed. RESULTS AND CONCLUSION： According to examination with naked eyes and X-ray, some of the segmental defects with the length of 12, 13, 14, 15 and 16 mm were completely repaired. But none of the defects with the length of 17 mm was repaired. Histological examination showed that there was trabecular bone formation, bone matrix, medullary cavity recanalization, revascularization and osteoblasts in the segmental defects with the length of 12, 13, 14, 15 and 16 mm. However, there were osteoblasts, osteoctasts in the 17 mm defects, but no medullary cavity recanalization or revascularization. Therefore, the length of bone critical-size defect in rabbit radius was 17 ram.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第50期9385-9388,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家高技术研究发展计划(863计划)(2006AA02A118)~~
