摘要
植物细胞脱壁酶 Cellulase ZA-P 是一种灰白色粉末,酶溶液的颜色极淡,透明,无沉淀,在45℃以下、pH4～7范围内其纤维素酶活力有较好的稳定性,粉剂贮藏1年以上其滤纸酶活力不变。Cellulase ZA-P 的滤纸酶活力为176.7IU/g,CMC 酶活力为1192.9IU/g,棉花酶活力为22.2IU/g,半纤维素酶活力为322IU/g,几丁质酶活力为4.0IU/g。果胶酶、蛋白酶、RNA 酶和过氧化氢酶的含量均很低,未测出α一淀粉酶酶活。制备原生质体的效果、物理性状以及酶系分析结果相似于日本 Yakult Honsha Co 的 Cellulase Onozuka R-10。CellulaseZA-P 对植物原生质体无毒害,适于制备各种植物的原生质体。
The properties of cellulase ZA-P preparation were examed.The preparation was fine powder of grey-white.Its 2% water solution was clearness with 80% transparency by spectrophotometry.No paperase activity loss was observed at the temperature of 4℃ for more than one year storage in its fine powder as well as under the condition of below 45℃ and pH 4～7 for 6 hours in its water solution.The concerned enzyme systems of cellulase ZA-P were measured.The paperase activity was 176.7IU/g,CMCase 1192.9IU/g,cottonase 22.2IU/g,hemicellulase 322IU/g and chitinase 4.0IU/g while pectinase,protease,RNA'ase,catalase and a-amylase were very low or not detected.Cellulase ZA-P could be apparently comparable to cellulase Onozuka R-10 in the isolation of plant protoplast,physical properties and enzyme activities.
出处
《浙江农业学报》
CSCD
1990年第1期41-45,共5页
Acta Agriculturae Zhejiangensis
