摘要
目的探讨姜黄素对脓毒症急性肝损伤的保护作用,为临床防治脓毒症提供新的治疗方法。方法将72只雄性SD大鼠随机平分成3组,即对照组(假手术组)、实验组(模型组)、干预组(姜黄素治疗模型组)。用盲肠结扎穿孔法制备脓毒症急性肝损伤大鼠模型。实验组和干预组接受盲肠结扎穿孔法处理,干预组大鼠制模1h按100mg/kg剂量腹腔内注射姜黄素,实验组和对照组予以等量生理盐水。手术后连续观察48h,选择术后6、12、24、48h为观察点。采用心脏采血测定肝功能丙氨酸氨基转移酶(ALT),肝组织匀浆测总抗氧化能力(T-AOC)、谷胱甘肽过氧化酶(GPx),并观察肝组织的病理变化。结果随着时间的变化,对照组ALT基本正常,实验组随着脓毒症严重程度增加ALT显著升高,姜黄素干预组ALT也升高,但升高幅度较实验组小,T-AOC和GPx在实验组较对照组表达均降低,T-AOC先升高后降低,而GPx逐渐升高,姜黄素干预后上调。对照组大鼠肝脏结构未见明显异常改变,实验组肝组织大量炎症细胞聚集、细胞肿胀,姜黄素干预后组织明显改善。结论姜黄素对脓毒症大鼠肝组织具有保护作用。
fObjective To investigate the protection of curcumin on acute liver InJury in sepsis and to provloe clinical a theoretical basis for the prevention and treatment of sepsis. Methods senventy-two rats were divided into three groups： control group（sham operation group）, experimental group （model group）and intervention group （curcumin treatment model group）. A rats model of liver injury in sepsis was prepared by method of cecal tigation and puncture. Experimental group and intervention group were given operation of cecal ligation and puncture. Intervention group was injected curcumin in abdominal at a dosage of 100 mg/kg one hour after mold. Same volume normal Saline was injected to the rats of Control group and experimental group. Continuous observation was carried on in 48 h after surgery, at 6,12,24,48 h which were the observation points. Alanine aminotransferase（ALT）of liver function was detected by taking cardiac blood. Total antioxidant capacity{T-AOC）and glutathione peroxidase（GPx） in liver tissue homogenate were deteeted. The pathological changes of liver tissue was observed. Results As time went on,the ALT of control group was normal, while significantly increased with the deterioration of sepsis in the experimental group. The ALT of curcumin intervention group increased, but was less than the experimental group. The expression of T-AOC and GPx decreased in the experimental group compared to the control group, T-AOC increased at first and then decreased, while GPx gradually increased, and both increased after curcumin intervention. The liver of control group rats showed no abnormal changes in the structure; hut there were a lot of inflammatory cells accumulation and ceils swelling in liver tissue of experimental group and they were improved after curcumin intervention. Conclusion Cureumin has a protective effect on liver tissue of septic rats.
出处
《成都医学院学报》
CAS
2011年第4期335-338,共4页
Journal of Chengdu Medical College
