摘要
本文提出了利用反相离子对色谱法,简单,快速,准确同时定量测定饲料添加剂中的烟酸,烟酰胺,盐酸吡哆辛,氰钴胺素,核黄素和硫胺素。样品前处理简单,六个维生素能在12min内获得满意的分离,各组份之间的分离度Rs大于1.60。各个维生素的回收率在87.0～101.0％,相对偏差为3.1～2.0％。色谱柱为LichrosorbRP-18 250×φ5mm,流动相为甲醇/水,0.25％TEA,1.25mmol/l PICB-5,3.75mmol/l PICB-7,pH3.00,紫外可变波长检测器采用MAXPL操作方式λ 1=254nm,λ_2=280nm,λ_3=354nm,流速1.1 nl/min。
A simple method is presented which accurately and rapidly quantitates niacin.niacinam-ide, pyridoxine, cyanocobolamin, riboflavin, and thiamine by reversed phase ion-pair liquid chromat-ography. Six vitamins can be separated satisfyingly, with all resolutions at above 1.61, recoveries in the range of 87.0%-101.0% and CV of 2.0<%-3.1%. The simplly pretreated sample is injected into the LC system consisting of a Lichrosorb RP-18 column, a mobile phase of water-methanol-acetic acid with 1.2GmM PIC B-5 + 3.T5mM PIC B-7 and o.25% TEA, flow rate 1.1ml/min and a UV detector Set at MAXPL mode with three wavelenths of 234 nm, 280nm and 354nm.
关键词
饲料添加剂
RPLC测定
B族维生素
reversed phase ion-pair liquid chromatography
B-group vitamins
feed additive
