摘要
目的:比较龟鹿二仙胶及其拆方含药血清对硝普钠(SNP)诱导后软骨细胞bax、bcl-2表达的影响,探讨龟鹿二仙胶的配伍机理。方法:取第三代4周龄SD大鼠关节软骨细胞,经SNP诱导凋亡后,采用免疫组化染色法及Realtime-PCR观察龟鹿二仙胶及其拆方含药血清对软骨细胞bax、bcl-2表达的影响。结果:龟鹿二仙胶及其拆方各组均可引起bcl-2表达升高及bax下降,与生理盐水组比较差异有统计学意义(P<0.05),龟鹿二仙胶与龟板、鹿角比较差异无统计学意义(P>0.05)。结论:龟板、鹿角、人参、枸杞均具有抑制软骨细胞凋亡的作用,而4味药同用,人参、枸杞并无显著加强龟板、鹿角的抑制凋亡作用。
Objective：To investigate the mechanism of GuiLu ErXian Glue in Rats＇chondrocyte apoptosis induced by nitroprussid,by detecting the expression of bax/bcl2in rats＇chondrocyte induced by nitroprussid treated with Guiluerxian glue and its serum reconstituent.Methods：Chondrocyte were extracted from rat＇s knee and cultured in vitro.Apoptosis of chondrocyte were induced by nitroprusside.The expressions of bax/bcl2were detected by immunohistochemistry stain and realtime-PCR.Results：The expression of Bcl-2was increased and the expression of Bax were decreased in the GuiLu ErXian group,there were significant difference in the expression of Bcl-2/Bax between the GuiLu ErXian group and the control group（P0.05）.There were no difference in the expression of Bcl-2/Bax between the GuiLu ErXian and the Guiban,Lujiao（P0.05）.Conclusions：Tortoiseshell,antler,ginseng and medlar can inhibit the apoptosis of chondrocyte in rat.Inhibition of apoptosis aroused by Tortoiseshell,antler was not strengthened by ginseng and medlar.
出处
《中国中医骨伤科杂志》
CAS
2012年第1期1-4,共4页
Chinese Journal of Traditional Medical Traumatology & Orthopedics
基金
国家自然科学基金(30772814)
福建省自然科学基金(2007J0098
C0710028)
福建省高等学校新世纪优秀人才支持计划(闽教科[2007]20号)资助
