摘要
针对芍药在组织培养过程中外植体污染难于控制的问题进行了研究。试验分别于不同的季节取材后对外植体进行不同的表面消毒处理。结果表明:以芍药带芽茎段、顶芽、侧芽为外植体,采用0.1%～0.2%HgCl2消毒8 min,结合母株黄化处理、4℃消毒液+搅拌的表面消毒处理,可降低外植体初期培养污染率;在培养基中添加抗菌素,可明显控制内生菌的污染。
To solve the problem of explant contamination during peony tissue culture,the explants of different seasons were studied.The results showed that soaking explants in 0.1%-0.2% HgCl_2 for 8min,etiolation treatment,low temperature and electronic vibration are effective for sterilization;Antibiotics added into the media can inhibit contamination caused by endophytes.
出处
《植物研究》
CAS
CSCD
北大核心
2012年第1期84-90,共7页
Bulletin of Botanical Research
基金
十一五国家科技支撑计划项目(2006BAD07B01)
关键词
芍药
外植体
组织培养
污染
抗菌素
peony
explant
tissue culture
contamination
antibiotics
