摘要
[目的]对草麻黄的子叶诱导出的愈伤组织,进行悬浮培养。[方法]采用组织培养的方法进行了愈伤组织诱导、愈伤组织继代和悬浮培养研究。[结果]MS+2.0 mg/L 2,4-D+1.0 mg/L 6-BA为诱导子叶形成愈伤组织的理想培养基;MS+1.5 mg/L 2,4-D+1.5 mg/L6-BA是愈伤组织的适宜继代培养基;2.0 mg/L 2,4-D+1.5 mg/L 6-BA+300 mg/L水解酪蛋白(CH)是愈伤组织悬浮培养的适宜培养基,愈伤组织干重增加量为0.80 g。[结论]初步选择出草麻黄愈伤组织细胞的悬浮培养条件,为麻黄细胞扩大培养及有效成分提取奠定基础。
[Objective] Ephedra callus induced with Ephedra explant were cultured in suspension culture.[Method] The conditions needed in callus induction,callus generation and suspension culture of Ephedrae sinica Stapf cotyledon were studied.[Result] The results showed that the best medium for callus induction was MS＋2.0 mg/L 2,4-D＋1.0 mg/L 6-BA.The best media of callus generation was MS＋1.5 mg/L 2,4-D＋1.5 mg/L 6-BA.The best media of suspension culture was 2.0 mg/L 2,4-D＋1.5 mg/L 6-BA＋CH 300 mg/L,increased amount of dry weight was 0.80 g.[Conclusion] The condition of Ephedrae sinica Stapf suspension culture was preliminary established,which will lay a foundation for the expanding culture and extracting active ingredient of Ephedrae sinica Stapf cell.
出处
《安徽农业科学》
CAS
2012年第3期1419-1420,共2页
Journal of Anhui Agricultural Sciences
基金
河北省教育厅课题(2008141)
河北省唐山市课题(09130202A-3-1)资助
关键词
草麻黄
愈伤组织
悬浮培养
Ephedrae sinica Stapf
Callus
Suspension culture
