摘要
目的探讨高压氧预处理是否可以通过抑制早期的细胞凋亡来保护脊髓后角神经元。方法随机将24只雄性Wistar大鼠分成对照组与高压氧预处理组。高压氧预处理组在给予高压氧5d后与对照组同时制作脊髓（T8-T10）全横断模型。术后8h、1d和3d取脊髓做冠状冰冻切片，行Nissl及TUNEL染色，光镜下观察。结果Nissl染色显示，术后8h及1d时脊髓后角内浓染的细胞多见，高压氧预处理组与对照组比较浓染的细胞较少，3d后两组无明显差异；TUNEL染色显示，术后8h及1d时阳性细胞多见，8h时两组差异明显（P〈0．05），1d时差异最显著（P〈0．01）。结论高压氧预处理对脊髓损伤后后角神经元起保护作用，术后1d内保护效果明显。
Objective To test the hypothesis that spinal cord posterior horn neurons are protected by hyperbaric oxygen preconditioning （HBO-PC） and mediated by inhibition of early apoptosis. Methods 24 Wistar male rats were randomly divided into 2 groups：control group and HBO-PC group. The rats in HBO-PC group were administrated HBO for 5 d,their spinal cords （T8-T10） were all transected as same as the control group. At 8 h, 1 d and 3 d after operation, the spinal cords were cut for coronal frozen sections, and the sections were stained with Nissl and TUNEL staining, they were observed by light microscope and analyzed by statistics. Results Nissl staining demon- strated that the hyperehromic cells were found in posterior horn neurons in two groups at 8 h and 1 d after operation, the hyperchromic cells were lesser in HBO-PC group than that of control group,no big difference between two groups at 3 d postoperative;TUNEL staining demonstrated that the positive cells were most found in posterior horn neurons at 8 h and 1 d after the operation, there were difference between two groups at 3 d postoperative. Statistic analysis demonstrated that the difference was obvious at 8 h （P〈 0.05） and significant at 1 d （P〈 0.01 ）postoperative, respectively. Conclusion The results demonstrated that HBO-PC could protect posterior horn neurons after spinal cord injury, the effects were shown most significant within 1 d after spinal cord injury.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2012年第1期11-13,17,共4页
Journal of China Medical University
基金
国家自然科学基金资助项目(30872669)
关键词
高压氧预处理
脊髓损伤
前角运动神经元
凋亡
hyperbaric oxygen preconditioning
spinal cord injury
posterior horn neuron
apoptosis
