摘要
目的探讨Notchl蛋白和基质金属蛋白酶2（MMP-2）、MMP-9在胶质瘤组织中的表达，及其与胶质瘤进展和预后的关系。方法采用免疫组化SP法检测64例胶质瘤组织和癌旁组织中Notchl、MMP-2和MMP-9的表达，并分析患者的牛存情况。结果Notchl、MMP-2和MMP-9蛋白在胶质瘤组织中均有表达，而在癌旁组织没有表达，其中Notchl的表达强度与病理级别呈正相关（P〈0．05）。Notchl强阳性表达组的患者中位生存时间（31．0个月）明显短于Notchl非强阳性表达组（53．0个月，P〈0．05），MMP-9阳性表达患者的中位生存时间（32．0个月）短于阴性表达者（110．0个月），差异有统计学意义（P〈0．05）。Notchl与MMP-2、MMP-9的表达均呈正相关（t=0．434；P〈0．01；rs=0．273，P〈0．05）。结论Notchl、MMP-2、MMP-9与胶质瘤的进展存在密切关系，Notchl可能参与MMP-2、MMP-9的调控。与MMP-2相比，MMP-9在影响胶质瘤患者预后方面起着更为重要的作用．MMP-9和Notchl可能成为判断胶质瘤预后的生物学指标。
Objective To study the expression of Notchl, MMP-2 and MMP-9 in glioma patients and their relationship with progression and prognosis of gliomas. Methods Sixty-four cases of glioma were included in this study. There were four cases of grade 1 tumor, twenty-five cases of grade 2, nine cases of grade 3, and twenty-six cases of grade 4. Inlmunohistochenlistry（SP staining method） was used to detect the expression of Notchl, MMP-2 and MMP-9 in glioma tissues and adjacent non-tumor tissues, and the patients were followed up. Results Notch1, MMP-2 and MMP-9 were detected in glioma tissues but not in adjacent non-tumor tissues. The expression of Notch1 was increased with the pathological grade of the gliomas （r = 0. 262, P 〈 0.05）. The survival time of patients with strong expression of Notch1 was 31.0 months, significantly shorter than that of patients with non-strong positive （negative, weak and moderately） Notchl expression （53.0 months, P 〈 0.05）. Significant difference in survival time was observed between patients with negative and positive expression of MMP-9 （ P 〈 0.05 ）. Conclusions Notchl, MMP-2 and MMP-9 are closely correlated with the progression and prognosis of malignant gliomas. Notchl may participate in the expression regulation of MMP-2 and MMP-9. Compared with MMP-2, MMP-9 may play a more important role in determining the prognosis of malignant glioma. Notehl and MMP-9 may become new biological markers for prognosis of patients with malignant glioma.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2012年第1期26-30,共5页
Chinese Journal of Oncology
基金
国家自然科学基金（30700253、30800355）
