摘要
为了提高球孢白僵菌CXJ-1(Beauveria bassiana CXJ-1)在液体发酵过程中分生孢子的产孢量,采用响应面法对球孢白僵菌CXJ-1产孢的发酵工艺条件进行优化。采用单因素实验确定了产孢发酵条件最适初始pH、发酵转速和发酵温度。通过Plackett-Burman实验设计和Box-Behnken实验设计响应面分析优化,确定了球孢白僵菌CXJ-1产孢最佳发酵工艺参数为:温度28.07℃,转速198.95r/min,初始pH 6.10,预测孢子浓度可达9.5×108个/mL,在优化发酵条件下进行验证实验,孢子浓度达到9.7×108个/mL,表明验证值和预测值有较高的吻合度。
Response surface methodology was used to optimize fermentation conditons in order to improve spore production of Beauveria bassiana CXJ-1.The optimum fermentation temperature,rotation speed and pH were obtained with one-factor-at-time.Through Plackett-Burman experimental design and Box-Behnken experimental design for response surface optimization,the optimal conditions for spore production of Beauveria bassiana CXJ-1 were also determined as follows：temperature 28.07 ℃,rotation speed 198.95 r/min and the pH value 6.10.Under these conditions,the predicted value of the concentration of spores was 9.5×108 spores/mL and the verified value was 9.7×108 spores/mL,the fermentation validation showed that the experimental value have high inosculating degree to model prediction.
出处
《石河子大学学报（自然科学版）》
CAS
2011年第6期689-695,共7页
Journal of Shihezi University(Natural Science)
基金
新疆兵团重大产学研专项(2010ZX03-1)
国家科技支撑计划项目(2011BAD48B02)
关键词
球孢白僵菌
响应面优化
发酵工艺条件
产孢
Beauveria bassiana
response surface optimization
fermentation conditons
sporulation
