摘要
为了分离新疆早实核桃上与花分生组织相关的基因,以早实核桃的花芽为实验材料,通过对不同植物的AP1保守区核苷酸序列分析,设计1对引物,采用RT-PCR的方法克隆获得1条长度为463bp的PCR产物,命名为JrAP1。该片段与其他植物的AP1同源基因序列同源性达70%～86%,推测所得的基因为AP1基因的同源基因对其全长和功能将进行进一步研究分析。初步RT-PCR分析的结果表明:JrAP1在早实核桃的顶芽、花芽、枝条、果实、老叶、嫩叶上都有表达,说明了该基因参与了早实核桃的营养生长和生殖生长。而且其在新疆早实核桃及晚实核桃上都能够得到表达,扩增产物亮度早实核桃>晚实核桃,推测其可能在早实机制上起到一定的作用。
In this experiment,we designed a pairs of primers according to the conservative regions of floralmeristem identity genes-APETALA1（AP1） homologous genes.About 463 bp fragment of this homologous gene was amplified by RT-PCR using the pair of primers from the genomic RNA of walnut.The result of blasting sequence indicates that the homology reaches 70%~86% of the other AP1 homologous gene.It was named JrAP1.We guess this fragment was the homologous gene of AP1,and we will research its function and the full length in further.Expression analysis of JrAP1 was detected in different tissue in precocious by RT-PCR,the result showed that this gene could be expressed with vegetative and reproductive growth.There were expression between later-seeding walnut and precocious walnut,but the expression in early-seeding was stronger than another,it was maybe related to the character of precocious.
出处
《石河子大学学报（自然科学版）》
CAS
2011年第6期679-682,共4页
Journal of Shihezi University(Natural Science)
基金
国家自然科学基金项目(30560090)
新疆维吾尔自治区重大专项(201130102-1-4)
