摘要
为建立一种能快速检测鸡传染性贫血病毒病(CIAV)的检测方法,根据基因库中鸡传染性贫血病毒病的保守序列,设计一套特异性环介导等温扩增(LAMP)引物,建立了CI-AV的LAMP可视化检测方法。该法敏感性可达10fg,高于常规PCR方法10倍;全部反应可在1h内完成;可通过肉眼观察颜色直接判定结果;对其它鸡常见病原体的检测结果均为阴性。结果表明建立的LAMP方法简便、快速、灵敏、特异,可用于CIAV感染的快速检测。
A loop-mediated isothermal amplification （LAMP） assay was developed for detection of chicken infectious anemia（CIAV）. According to the sequences of CIAV in GenBank, six primers were designed, and the reaction conditions were optimized. The results showed that the detection limit of this LAMP method was 10 fg, which was higher than the routine PCR. The amplification could be finished within lh, and the presence of CIAV could be detected by naked eyes. There was no cross-reactivity with the other pathogen of chiken. These results suggest that this LAMP assay is a simple and specific method for rapid detection of CIAV in clinical samples.
出处
《家畜生态学报》
2011年第6期57-60,共4页
Journal of Domestic Animal Ecology
基金
广西科技攻关项目(桂科攻0537008-3A
桂科攻1123007-4)
广西特聘专家专项经费(2011B020)
新世纪百千万人才工程国家级人选专项经费(945200603)
