摘要
根据已克隆的刺五加鲨烯合酶(squalene synthase,SS)、鲨烯环氧酶(squalene epoxidase,SE)和β-香树酯醇合成酶(β-amyrin synthase,bAS)基因序列信息设计引物,通过半定量RT-PCR分析了SS、SE和bAS基因在刺五加不同生长发育时期和不同器官中表达量的变化。结果表明,SS、SE和bAS基因在各生长发育时期和各器官中均有表达,但表达量差异显著(P<0.05),三者均在盛花期表达量最高,之后降低,进入果实成熟期后SS和bAS的表达量迅速回升,SE无显著变化。SS和bAS在叶片和根中的表达量较高,SE表达量的最大值出现在叶片和幼茎中。刺五加SS、SE和bAS基因的表达间存在显著的正相关关系(P<0.05)。研究结果为进一步分析关键酶基因对刺五加三萜皂苷生物合成的影响奠定了基础。
Primers were designed according to available sequence information of the cloned SS （squalene synthase）, SE （squalene epoxidase） and bAS （β-amyrin synthase） genes. Semi-quantitative RT-PCR was developed to analyze expression variations of SS, SE and bAS genes during different growth and development stages and in different organs. Semi-quantitative RT-PCR results showed that SS, SE and bAS genes were expressed but their expression levels had significant differences （P〈0.05） during different growth and development stages and different organs. Those three genes all had highest expression levels during full flowering stage, and then the expression levels decreased. After entered into the maturation stage, the expression of SS and bAS increased rapidly, but SE showed no significant difference. The expression levels of SS and bAS were higher in blade and root compared with other organs. The highest expression value of SE was in blade and tender stems. The expression value among SS, SE and bA S had significantly positive correlation （P〈0.05）. The result provided a foundation for further analysis on key enzyme genes which effect on the biosynthesis oftriterpenoid saponins in EIeutherococcus senticosus.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2011年第6期691-696,共6页
Genomics and Applied Biology
基金
国家自然科学基金(30701086)
河北省自然科学基金(C2009001252)共同资助
