摘要
目的观察连续给予香加皮不同组分导致大鼠慢性毒性的损伤表现、程度及可逆性。方法分别给140只大鼠灌胃高、中、低剂量的香加皮水提组分和醇提组分样品,除观察一般状况外,检测血常规、血生化指标,剖杀大鼠,精密称取心、肝、脾、肺、肾脏等重要脏器,计算脏、体比值,进行常规病理检查。停药后,进行恢复期观察。结果连续20天给予不同剂量的香加皮水提组分和连续9天给予不同剂量的醇提组分样品均可导致大鼠体重下降,饮食、饮水不佳,血ALT、AST、AKP、TPC增高,ALB、CR降低、A/G比值降低,肝脏重量和肝体比值、肾脏重量和肾体比值增大,病理检查可见不同程度的肝脏、肾脏病理组织损伤;对血常规影响不明显;肝、肾毒性损伤程度与给药剂量呈现一定的剂量依赖相关性;经过恢复期观察,上述部分病变不可逆。结论香加皮水提组分给药20天、醇提组分给药9天造成的慢性毒性损伤部位以肝、肾损伤为主,且部分病变为不可逆性损伤。
Objective The obvious toxic symptoms and signs, the toxic damage extents and reversibility were observed in rats caused by different components of Cortex Periplocae R.adicis. Methods The 140 rats were divided into seven groups such as normal group、water extract and alcohol extract components of high, medium and low dose. The general including the bodyweight,diet and behavior were observed , the blood routine, the biochemical indicators, organ/bodyweight rate ,the important organs such as heartdiver、spleenjung、kidney、brain and et al. and pathological changes were detected aider 20 days water extract and 9 days alcohol extract components administrated, and then we continued to observe the recovery period after drug discontinuance. Results The Wistar rat＇s weight,diet and drinking were decreased; the serum ALT,AST,AKP,TPC were increased; the ALB、CR and the rate of A/G were decreased; the liver weight and volume ratio,kidney weight and volume ratio were increased after given water extract and alcohol extract components from Cortex Periplocae R.adicis to Wistar rats by intragastric for 21 and 9 days. Pathological examination showed different degree of liver and kidney pathology tissue damage; There was little affect for routine blood; the damage of liver and kidney toxicity in rats show an obvious dosage relationship, and after the convalescence, the partial lesions were irreversible. Conclusion The main chronic toxical damage is hepatic and kidney injury in rats caused by the 20 days water extract and 9 days alcohol extract components of Cortex Periplocae P.adicis, and part of the lesions were irreversible.
出处
《中国药物警戒》
2012年第1期9-15,共7页
Chinese Journal of Pharmacovigilance
基金
国家重点基础研究发展计划(973)中医基础理论专项资助项目(2009CB522802)
山东省科技平台建设项目课题(2008GG2NS02021)
关键词
香加皮
不同组分
大鼠
长期毒性
Cortex Periplocae Radicis
different components
rats
chronic toxicity
