摘要
目的研究再生障碍性贫血患儿外周血淋巴细胞亚群分布,及其与同期外周血血常规的相关性。方法采用流式细胞技术对我院42例AA患儿和27例正常儿童外周血淋巴细胞亚群CD3^+,CD3^+CD4^+,CD3^+CD8^+,CD3^+CD4^+/CD3^+CD8^+,CD3^+CD45RA^+,CD3^+CD45RO^+,CD(16+56)^+,CD19^+的表达进行检测,同时检测血常规。结果再障组CD3^+CD4^+,CD3^+CD8^+,CD3^+CD4^+/CD3^+CD8^+的平均值分别为(26.94±11.93)%,(33.33±10.3)%,0.918±0.53,对照组分别为(33.53±9.97)%,(27.08±9.23)%,1.44±0.80,差异有显著性(P<0.05);CD3^+CD8^+与HB,WBC呈一定程度的正相关,CD3^+CD4^+/CD3^+CD8^+比值与HB,WBC,N呈一定程度的负相关。结论再障患儿存在淋巴细胞亚群的失调,其中由T淋巴细胞介导的免疫异常对造血功能的抑制在再障的发生中起重要作用,再障患儿Th格局向Th1偏移。
Objective To investigate the distribution of lymphocyte subsets in peripheral blood and its correlation with complete blood count in children with aplastic anemia.Methods The lymphocyte subsets CD3~＋,CD3~＋ CD4~＋,CD3~＋ CD8~＋,CD3~＋ CD4 VCD3~＋ CD8~＋,CD3~＋ CD45RA~＋,CD3~＋ CD45RO~＋,CD（16 ＋56）~＋,CD19~＋from 42 aplastic anemia children and 27 controls were examined by flow cytometry;its correlations with complete blood count were analyzed.Results CD3~＋ CD4~＋ and CD3~＋ CD4 VCD3~＋ CD8~＋ decreased while CD3~＋ CD8~＋ increased in aplastic anemia patients.CD3~＋ CD8~＋ was positively correlated with peripheral HB,WBC counts.CD3~＋ CD4~＋/CD3~＋ CD8~＋ was negatively correlated with peripheral HB,WBC and N counts.Conclusion There are abnormal distributions of lymphocyte subsets in aplastic anemia patients,which may be involved in pathogenesis of the disease.
出处
《同济大学学报（医学版）》
CAS
2011年第6期70-73,共4页
Journal of Tongji University(Medical Science)
关键词
贫血
再生障碍性
淋巴细胞亚群
血常规
儿童
aplastic anemia
lymphocyte subsets
complete blood count
children
