摘要
目的:观察干扰素α-2a(interferon alfa-2a,IFNα-2a)对CCl4诱导肝纤维化的作用及影响因素.方法:建立CCl4诱导大鼠肝纤维化模型,SD雌性大鼠50只,分成5组,每组10只,即生理盐水对照组(A组)、纤维化模型组(B组)、6×104U/kgIFNα-2a干预组(C组)、12×104U/kgIFNα-2a干预组(D组)及6×104U/kgIFNα-2a对照组(E组).造模8wk时采集血标本及肝组织标本,分别进行肝功能指标ALT、AST、TBIL、TP,肝纤维化指标HA、LN、PCIII检测,及组织病理形态学观察包括HE染色、Masson染色和网状纤维染色.结果:CCl4腹腔注射成功诱导大鼠肝纤维化模型,表现为汇管区周围纤维化明显,有芒状纤维和纤维间隔形成.血清学检测:B、C、D组ALT、AST、TBiL、HA、LN均明显高于A组(F值分别为14.8,4.4,7.8,51.3,68.9;均P<0.05);C、D组ALT、AST、TBiL均明显低于B组;D组的上述指标又明显低于C组.组织病理:HE染色、Masson染色和网状纤维染色均显示,C、D组肝组织炎症及肝纤维化程度较B组显著减轻,D组较C组肝纤维化程度更轻,A、E组肝组织未见炎症及纤维化.结论:IFNα-2a能够阻断CCl4诱导肝纤维化,其作用效果随IFNα-2a剂量增加而增强.
AIM：To observe the effect of IFNα-2a on liver fibrosis induced with carbon tetrachloride（CCl4） in rats.METHODS：Fifty female Sprague-Dawley ratswere equally and randomly into five groups：groups A,B,C,D and E.Liver fibrosis was induced in rats of groups B,C and D with CCl4.Group A was treated with normal saline,while groups C,D and E were treated with 60 000,120 000 and 60 000 U/kg IFNα-2a,respectively.At week 8,blood and liver tissue samples were collected to measure liver function（ALT,AST,TBIL,TP） ,evaluate liver fibrosis（HA,LN,PCIII） and pathological changes（HE staining,masson staining and reticular fiber staining） .RESULTS：Hepatic fibrosis was successfully induced by CCl4 injection,and significant fibrosis around the portal area and formation of spiky fibers and fibrous septa were observed.Serum levels of ALT,AST,TBiL,HA and LN levels were significantly higher in groups B,C and D than in group A（F=14.8,4.4,7.8,51.3,68.9;all P0.05） .Serum levels of ALT,AST and TBiL were significantly lower in groups C and D than in group B,and in group D than in group C.CONCLUSION：IFNα-2a reduces CCl4-induced liver fibrosis in a dose-dependent manner in rats.
出处
《世界华人消化杂志》
CAS
北大核心
2011年第31期3207-3211,共5页
World Chinese Journal of Digestology
基金
江西省科技厅基金资助项目
No.2009BSB11115~~
