摘要
【目的】通过动物实验探讨子宫动脉栓塞术(UAE)对子宫内膜整合素β3表达含量的影响。【方法】48只健康雌性豚鼠随机分为对照组(n=12)及UAE组(n=36);UAE组内再随机分为A1、A2及A3三个亚组(每组n=12)。UAE组动物应用三丙烯微球(Embosphere,40～120μm及100～300μm)行双侧子宫动脉栓塞术。A1、A2、A3组动物分别于UAE后16～30 d、31～45 d及46～60 d内获取子宫标本。各组动物均于排卵日后3 d获取子宫标本。应用免疫组化法检测各组标本内膜整合素β3的表达含量;以平均光密度值(MOD)代表阳性染色区域的平均染色强度,作为反映内膜β3含量的指标。同时在取标本时检测各组动物的血清雌孕激素水平。【结果】各组动物标本内膜整合素ανβ3的MOD值分别为0.399±0.038(对照组);0.268±0.035(A1组);0.321±0.038(A2组);0.366±0.043(A3组)。对照组整合素β3的MOD值均明显高于A1、A2及A3组(P1<0.01;P2<0.01;P2<0.05);而A1组与A2组、A2组与A3组之间的MOD值比较也有统计学差异,A2组高于A1组(P<0.01),A3组则高于A2组(P<0.01)。各组动物的血清雌孕激素水平则无统计学差异(P>0.05)。【结论】UAE后子宫内膜整合素β3含量在一定时期内有所下降,从而可能对内膜容受性造成一定程度的负面影响。随着栓塞后时间推延,β3含量有逐渐恢复趋势。
[Objective] To explore the impact of uterine artery embolization （UAE） upon the quantity of endometrial integrin 133 by experiment. [ Methods ] Forty-eight female guinea pigs were randomly divided into control group （n = 12） and UAE group （n = 36, further divided into A1, A2 and A3 subgroup, 12/subgroup）. The UAE group was performed bilateral uterine artery embolization with trisacryl gelatin microspheres （Embosphere, 40-120 μm and 100-300μm）. All the animals were sacrificed and obtained uterine specimens 3 days after ovulation day. The A1, A2, and A3 group were obtained specimens within 16-30, 31-45, and 46-60 days, respectively after UAE. The levels of endometrial integrin β3 were evaluated by immunohistoehemistry assay. The MOD （mean optical density） value of positive cells in endometrial epithelium was used as β3 level index. The serum estrogen and progestogen levels were measured for all groups specimens collection. [ Results ] The MOD value of endometrial integrin β3 for the control group and UAE group were 0.399 _＋ 0.038 （control group）, 0.268 _＋ 0.035 （A1 subgroup）, 0.321_＋0.038 （A2 subgroup） and 0.366 _＋ 0.043 （A3 subgroup）, respectively. The statistical analysis indicated MOD values of 133 levels in control group were significantly higher than those in A1, A2, and A3 subgroup, respectively （P1 〈 0.01, p2 〈 0.01, p3 〈 0.05）; and they were significantly higher in A2 subgroup than in A1 subgroup （P 〈 0.01 ）, also in A3 subgroup than in A2 subgroup （P 〈 0.01 ）. The serum level of estrogen and progestogen between all groups were not statistically significant different （P 〉 0.05）. [Conclusion] The level of integrin β3 in endometrium would decrease after UAE and it might lead to negative influence on endometrial receptivity in some extent. With time prolonged after embolization, the level of β3 might have the trend to recover gradually.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2011年第6期747-751,共5页
Journal of Sun Yat-Sen University:Medical Sciences
基金
广东省自然科学基金(S2011010006028)
关键词
子宫动脉
栓塞
内膜容受性
整合素
uterine artery
embolization
endometrial receptivity
integrin
