摘要
目的:研究巨核细胞多倍体细胞周期调控的机制。方法:Western blot分析多倍体细胞模型中mTOR/p70s6k通路信号分子表达和磷酸化修饰位点的变化,流式细胞仪双荧光分析S6K1不同结构域磷酸化位点修饰与细胞周期各时相的关系。结果:诺考达唑诱导的Dami细胞可作为相对同步化的多倍体细胞周期模型,mTOR表达增加及第2448位丝氨酸位点磷酸化,发生在G1期进入S期,S6K1的第421位苏氨酸/第424位丝氨酸位点磷酸化发生在G2/M期。结论:mTOR/S6K1通路参与巨核细胞多倍体细胞周期的调控。
AIM： To investigate the mechanisms of megakaryocyte polyploid cell cycle control. METHODS. The expression and phosphorylation of mTOR/p70s6k path- way proteins was detected by western blot. Double-labeling techniques were used to investigate in which of the phase of the polyploid cell cycle S6K1 at Thr421/Ser424 are phospho- rylated. RESULTS. Nocodazole induced a relatively syn- chronized polyploidization in Dami cells. The expression of mTOR and the phosphorylation of mTOR at Ser2448 increased when Dami cells begin to progress from G1 to S- phase in cell cycle. Analysis of flow cytometry showed that phosphorylation of S6K1 at Thr421/Ser424 increased at G2/ M-phase. CONCLUSION： mTOR/S6K1 pathway is in- volved in megakaryocyte polyploid cell cycle control.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2012年第1期21-24,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
辽宁省自然科学基金资助项目(20062079)
