摘要
目的:研究培养液pH值、培养时间及培养温度对大豆发芽过程中多肽富集的影响。方法:通过响应面试验法对培养条件进行优化,确定发芽大豆多肽最优富集工艺。结果:培养液pH值、培养时间及培养温度对发芽大豆中的多肽含量变化影响显著。结论:大豆最适培养条件为:以pH5.2的柠檬酸-柠檬酸钠缓冲液(5mmol/L)作为培养液、培养温度29.0℃、培养时间6d,在此条件下,发芽大豆多肽含量为159.38mg/g,是原料大豆中的3.8 3倍。
Objective： To explore the effect of pH,culture time and culture temperature on polypeptide accumulation in soybean during germination.Methods： Culture conditions optimization for maximizing polypeptide accumulation was performed using response surface methodology based on Box-Behnken experimental design.Results： All the investigated conditions were found to significantly affect the content of polypeptides during the germination process of soybean.Conclusion： The optimal conditions for the accumulation of polypeptides in germinated soybean were achieved after 6 days of culture at 29.0 ℃ in 5 mmol/L,pH 5.2 CBS buffer.The optimized culture resulted in a polypeptide content of 159.38 mg/g soybean,revealing a 3.83-fold increase in compared with non-germinated soybeans.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第23期172-175,共4页
Food Science
基金
江苏省科技支撑计划项目(BE2010386)
关键词
大豆
发芽
多肽
富集
soybean
germination
polypeptide
accumulation
