摘要
以双酚A(BPA)为模板分子,以丙烯酰胺为功能单体,季戊四醇三丙烯酸酯为交联剂,采用悬浮聚合法,在水性体系中制备了表面带孔的分子印迹聚合微球(MIPM),制备的BPA分子印迹聚合微球(BPA-MIPM)吸附平衡时间为110 min,饱和吸附量为34.38μmol.g-1.BPA-MIPM以苯酚为竞争分子,分离因子达到22.88;制备的MIPM具有良好的选择吸附性能和良好的再生性能.以BPA-MIPM为分子识别物,基于在pH为1的酸性介质中,β-环糊精对双酚A荧光强度的增强作用,建立测定BPA的分子印迹-流动注射分子荧光法.测得BPA在0.1～100μg.L-1线性范围内具有很好的相关性,相关系数r为0.9993,相对标准偏差(20μg.L-1,n=11)为0.79%,检出限为0.08μg.L-1,用该方法测水中BPA的加标回收率为98.6%～103.9%,结果较好.
Using Bisphenol A （BPA） as the template, acrylamide as monomers, Pentaerythritol Triacrylate PETA as crosslinking agent,we synthesized MIPM in aquosity system wJith suspension polymerization, on which surface there are some holes. The absorbance of MIPM to hydroquinone will no longer increased after 110 minutes, it means that it reaches the saturation which were 34.38 μmol · L^- 1. The separate genes of MIPM ,was 22.88 with phenol as competitiors ; The MIPM had the better effect of adsorption and selectivity. Using BPA - MIPM as molecule recognizates, Based on the enhancing effect of β - cyclodextrin on the flu- orescence from BPA in acidic solution, we set up a new method which was molecular imprinted technique - flow injection - che- analysis to detect hydroquinone. The result shows that the range of hydroquinone concentration from 0.1 μg · L-1 to 100 μg · L-1 with adsorbed by MIPM is linearly dependent,which relativity coefficient is 0.9993and the relative standard deviation is 0.79% （20μg · L-1 ,n = 11 ） ,the detection limit is 0.08μg · L-1. Using the method to detecting hydroqui- none from the water sample,the recoveries of hydroquinone is 98.6% -103.9% ,the result is pretty good.
出处
《上海师范大学学报（自然科学版）》
2011年第6期574-579,共6页
Journal of Shanghai Normal University(Natural Sciences)
关键词
分子印迹聚合微球(MIPM)
双酚A
悬浮聚合
吸附时间
分离因子
分子荧光
Β-环糊精
molecular imprinted polymer microsptleres（MIPM）
bisphenol A
suspension polymerization
adsorption time
separate genes
molecular fluorescence
β- cyclodextrin
