摘要
[目的]分析深圳本地12种宝巾种质资源的遗传多样性。[方法]利用聚丙烯酰胺凝胶电泳法研究宝巾种质资源过氧化物同工酶(POD)和酯酶同工酶(EST)的遗传多样性,并对统计结果进行聚类分析。[结果]各宝巾种质资源间有丰富的遗传多样性,在相似系数为0.63水平上可以聚为2大类。[结论]同工酶的特征谱带应用于品种鉴定是可行的,在一定程度上明确了各宝巾品种的亲缘关系。
[Objective] The aim was to analyze the genetic adversity of 12 Bougainvillea spp.germplasm resources from Shenzhen region.[Method] The esterase（EST） isozyme and peroxidase（POD） isozyme of Bougainvillea spp.germplasm resources were determined by polyacrylamide gel electrophoresis and the cluster analysis was used according to statistical results.[Result] There was great genetic adversity among different germplasm resources and the resources could be divided into two groups based on 0.63 of similarity coefficient.[Conclusion] The characteristic band of isozyme was suitable for variety identification and defining the blood relationship for various varieties to some extent.
出处
《安徽农业科学》
CAS
2012年第1期29-31,共3页
Journal of Anhui Agricultural Sciences
基金
深圳市科技局基础研究项目(JC200903190758A)
关键词
宝巾
遗传多样性
同工酶
过氧化物酶
酯酶
聚类分析
Bougainvillea spp.
Genetic diversity
Isozyme
Peroxidase（POD）
Esterase（EST）
Clustering analysis
