摘要
以慈竹、龙竹和紫竹3个竹种为研究对象,通过对竹子的新鲜叶片中的rDNA的ITS序列进行聚合酶PCR扩增,以及对其进行序列测定及分析。检测出目的片段ITS总长分别为慈竹390 bp、龙竹384 bp、紫竹380 bp。3种不同属的竹种之间的ITS序列有差异。同时在国家生物多样性保护信息系统(NCBI)上选取同属竹种ITS序列进行比较分析。以此建立系统树,用以分析它们之间的亲缘进化关系。
Using the fresh leaves of Neosinocalamus affinis,Dendrocalamus giganteus,Phyllostachys nigra as research materials,the extraction of DNA was done,and the genes of Internal Transcribed Spacer were amplified from genomic DNA by polymerase-chain-reaction（PCR）.Then the PCR products were sequenced and analyzed.The lengths of the PCR production were 390 bp,384 bp,and 380 bp for N.affinis,D.giganteus,and Phy.nigra respectively.The sequences are different to each other.Meantime,other ITS sequences of the genus in the NCBI were selected.Then the phylogenetic tree was established on the basis of the ITS sequences in order to analyze their evolutionary relationships.
出处
《四川林业科技》
2011年第6期73-75,共3页
Journal of Sichuan Forestry Science and Technology
基金
西南生物多样性保育国家林业局重点实验室开放基金(BC2010F07)
国家林业局918项目(2008-4-30)
云南省重点学科(西南林业大学森林培育)资助
关键词
竹种
内转录间隔区(ITS)
序列分析
系统树
Bamboo species
Internal Transcribed Spacer
Sequencing analysis
Phylogenetic tree
