摘要
利用32对SSR引物对湖南省茶叶研究所选育的16个茶树品种(系)进行了遗传多样性和遗传结构分析。结果表明:32对引物共检测到等位位点88个,每个引物为2～4个,平均2.75个;遗传多态性信息含量(PIC)在0.12～0.80之间,平均值为0.55。观测杂合度(Ho)在0.11～0.94之间,平均值为0.58;期望杂合度He大小在0.25～0.84之间,平均值为0.62;群体的Shannon指数最小为0.40,最大为1.88,平均值为1.11。湖南省茶叶研究所选育的茶树品种(系)间具有相对较高的遗传多样性。基于SSR数据以SAHN邻接法对供试品种(系)进行UPGMA遗传相似性聚类,并绘制树状聚类图,按相似系数为0.55可将参试的16份茶树品种(系)分为三大类。
The 32 pairs of SSR primers were used to analyze the genetic diversity and genetic structure of 16 tea cultivars bred by Hunan Academy of Agriculture Sciences. The results showed that totally 88 alleles were amplified by using 32 pairs of SSR primers, namely the number of alleles per primer ranged from 2 to 4 on average of 2.75; the polymorphism information content （PIC） was from 0.12 to 0.80 on an average of 0.55; the observed heterozygosity （Ho） ranged from 0.11 to 0.94 and the expected heterozygosity （He） varied from 0.25 to 0.84, which on average of 0.58 and 0.62, respectively; the Shannon index of the population varied from 0.40 to 1.88 on a average of 1.11. Therefore the genetic diversity of the 16 tea cultivars was relatively abundant. The 16 cuhivars were classified into three groups based on the UPGMA method with the similarity coefficient at 0.55.
出处
《湖南农业科学》
2011年第12期6-9,共4页
Hunan Agricultural Sciences
关键词
茶树
遗传多样性
遗传结构
SSR
tea tree
genetic diversity
genetic structure
SSR
