摘要
目的 了解我国结核分支杆菌耐吡嗪酰胺 (PZA)分离株pncA基因突变情况 ,研究其与耐PZA之间的关系。方法 通过聚合酶链反应 (PCR) -单链构象多态性 (SSCP)和PCR -循环测序法(AS)分析 74株结核分支杆菌临床分离株的pncA基因。以结核分支杆菌H37RV 标准株为对照。结果 3 2株药物敏感株pncA基因SSCP分析未发现异常。 2 0株耐非PZA药物的分离株中 ,16株pncA基因SSCP泳动正常 ;4株异常 ,其中 2株测序分析分别为 5 8位密码子TTC→TTA突变和 68位密码子TGG→CGG突变。 2 2株耐PZA分离株中 ,10株pncA基因SSCP泳动异常 ,其中 2株测序分析均为 5 8位密码子TTC→TTA突变 ;12株未发现异常。结论 PncA基因突变是结核分支杆菌耐PZA产生的一种分子机制 ,通过检测pncA基因突变可快速检测部分结核分支杆菌耐PZA基因型。
Objective To understand the mutations of pncA gene in M tuberculosis isolates, and to evaluate their clinical value Methods Analyzing the pncA genes in 74 M tuberculosis clinical isolates with PCR SSCP and PCR AS M. tuberculosis strain H 37 R v was used as control Results 32 drug sensitive isolates all displayed normal pncA SSCP profile Of 20 non pyrazinamide resistant isolates, 4 had abnormal pncA SSCP profile, in which 2 isolates were sequenced, one was TTC→TTA mutation at codon 58, another was TGG→CGG mutation at codon 68 Of 22 pyrazinamide resistant isolates, 10 displayed abnormal pncA SSCP profile, in which 2 isolates sequenced had TTC→TTA mutation at codon 58 Conclusions The mutation of pncA gene is a pyrazinamide resistant molecular mechanism in M tuberculosis Detecting the mutations of pncA genes might diagnose pyrazinamide resistance rapidly in some M tuberculosis isolates
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
2000年第1期40-42,共3页
Chinese Journal of Tuberculosis and Respiratory Diseases
关键词
分支杆菌
药物耐受性
吡嗪酰胺
肺结核
Mycobacterium tuberculosis
Drug resistance
Polymerase chain reaction
Polymorphism, single stranded conformation
DNA sequencing
