摘要
目的探讨全反式维甲酸(at-RA)对单侧输尿管梗阻大鼠(UUO)肾间质纤维化的干扰。方法将30只大鼠随机分为假手术组、模型组、给药组,结扎单侧输尿管造成梗阻性肾病模型。术后9天处死大鼠,取梗阻侧肾做组织学检查,HE染色,用免疫组化方法检测肾组织中结缔组织生长因子(CTGF),α-平滑肌肌动蛋白(α-SMA)。结果维甲酸显著减轻肾小管间质损害,治疗组肾间质炎细胞浸润和肾间质CTGF、α-SMA表达显著低于对照组。结论维甲酸减轻UUO模型大鼠肾小管间质损害和炎细胞浸润,降低CTGF表达,抑制α-SMA表达,从而减轻肾间质纤维化。
Objective To investigate the effct of retinoic acid on renal interstitial fibrosis ina rat unilateral ureteral obstruction model. Methods Thirty SD rats were randomly assigned into sham-operated, UUO groups, all-trans-retinoic acid treatment group. The tubulointerstitial fibrosis model was established by unilatered ureteral obstruction. These rats were sacrificed at 9 days after UUO or sham-surgery. The morphological changes was obsvered. The expression of CTGF,α - SMA in the obstructed kidney was assessed by immunchistachemistry methods. Results We obverse that there are the obvious tubulo dilatation and epidermis cell α- rophy and infiltration of the renal parenchyma with macrophages/monocytes and tubulointerstitial fibrosis in UUO groups. These changes are remarkably lighten in all-trans-retinoic acid treatment group. Conclusion all-trans-retinoic acid may suppress interstitial fibrosis by effect CTGF,suppress cell proliferation,redue extracellular matrix accumulation.
出处
《中国老年保健医学》
2011年第6期24-26,共3页
Chinese Journal of Geriatric Care
关键词
维甲酸
肾间质纤维化
结缔组织生长因子
Α-平滑肌肌动蛋白
all-trans-retinoic acid, tubulointerstitial fibrosis, college - Ⅳ, connective tissue growth factor, α - smooth musule active
