摘要
气单胞菌(Aeromonas)是一类人和水产经济动物的严重致病菌,本文从青岛某中华鳖养殖场不明原因死亡病例的病灶部位分离一株病原菌HBJY01,对该菌进行鉴定。采用传统鉴定方法结合HBJY01的16S rRNA和gyrB基因克隆的方法,鉴定该菌的属种(Aeromonas)。结果表明传统鉴定方法与分子生物学方法鉴定结果一致。人工感染实验结果表明HBJY01感染健康中华鳖,死亡率100%,症状与不明原因死亡的中华鳖大体相同。取其病变肝脏分离到相同病原菌。
Aeromonas as well as a serious pathogen of human and aquatic economic animals.this article from a turtle farm in Qingdao unexplained deaths of a pathogen isolated lesion site HBJY01,identification of the bacterium.Combination of traditional identification methods using the 16S rRNA and gyrB HBJY01 cloning method to identify the bacteria species.The results show that traditional identification methods and identification of molecular biology methods were consistent.Experimental results show that artificial infection HBJY01 infect healthy turtle,the mortality rate of 100%,symptoms and unexplained death of roughly the same turtle.Whichever is the same pathogen isolated from the liver lesions.
出处
《水产学杂志》
CAS
2011年第4期33-36,共4页
Chinese Journal of Fisheries
关键词
分离
鉴定
分子分类学
separation
identification
molecular taxonomy
