摘要
目的:研究探讨自然抗性相关性巨噬细胞蛋白1(NRAMP1)基因在宁夏人群类风湿性关节炎中的作用。方法:应用聚合酶链反应-限制性片段长度多态性分析(PCR-RFLP)法检测NRAMP1基因3’UTR、D543N两个多态性位点的基因型,进行类风湿性关节炎发病危险因素病例对照研究。结果:(1)NRAMP1-3’UTR位点TGTG+/del基因型在病例组中的分布频率显著高于对照组(P=0.039),差异具有显著性意义;3’UTR位点TGTGdel/del基因型在病例组和对照组中的分布差异无显著性意义(P=0.423)。(2)NRAMP1-D543N位点GA基因型在病例组中的分布无显著性意义(P=0.835);D543N位点AA基因型在病例组和对照组中的分布差异无显著性意义(P=0.722)。结论:NRAMP1-3’UTR位点与类风湿性关节炎的易感性具有相关性。
Objective：In the current study,the polymorphisms in the genes of NRAMP1 was examined to assess their contribution to Rheumatoid arthritis.The two site polymorphisms of candidate gene including NRAMP1-3＇UTR andD543N were involved in this study.Methods：Hospital-based case-control study design was adopted.Polymerase Chain Reaction（PCR） and Restriction Fragment Length Polymorphism（RFLP） technique were used to type polymorphisms of NRAMP1-3＇UTR and D543N.Results：（1）Univariate analysis demonstrated that the NRAMP1-3＇UTR-TGTG＋/del genotype occurred more frequently in the cases than in the controls（P=0.039）.No significant association was observed between RA and the genotype of NRAMP1-3＇UTR-TGTG del/del（P=0.423）.（2）No significant differences of D543N-GA variants were found between the cases and the controls,（P=0.835）while no significant association was found between RA and the genotype of D543N-AA（P=0.722）.Conclusion：The NRAMP1-3＇UTR-TGTG＋/del genotype may be associated with RA.
出处
《长治医学院学报》
2011年第6期411-414,共4页
Journal of Changzhi Medical College
基金
教育部"春晖计划"合作科研项目(Z2008-1-75019)
宁夏卫生厅科研项目(2008029)
宁夏医科大学校级科研项目(XM200828)
