摘要
为评价撕裂蜡孔菌P2处理橙黄G染料废水的应用潜力,采用批次实验在开敞系统中研究静置与摇动、染料初始浓度、pH、温度、盐度、碳源、氮源、金属离子等因子对该菌降解橙黄G染料废水的影响,同时利用植物萌发与微生物抑菌试验进行染料与脱色溶液的毒性测试.结果表明,与摇动培养相比,静置培养更适合于撕裂蜡孔菌的脱色,最适脱色pH与温度分别为9和25℃.盐度测试结果显示撕裂蜡孔菌能在浓度为128 g L-1的盐溶液中能进行高效脱色,可达70%以上.在上述参数体系的优化基础上,分别进行了碳源、氮源与金属离子的添加优化实验,结果显示碳源、氮源与金属离子的最适浓度分别为4 g L-1葡萄糖、0.15 g L-1硝酸铵和0.1 mmol L-1 Zn2+.菌丝吸附在整个脱色过程中作用较小,撕裂蜡孔菌对橙黄G的脱色过程以酶的降解为主,未发现该菌分泌漆酶,只分泌锰过氧化物酶与木质素过氧化物酶,其最高活性分别为230 U mL-1和158 U mL-1.植物与微生物毒性分析显示撕裂蜡孔菌脱色后的产物对植物与微生物的毒性大大降低.因此,撕裂蜡孔菌对于处理橙黄G染料废水具有良好的应用潜力.
In order to evaluate the application potential of Ceriporia lacerate P2 in treating dye wastewater, batch experiments were carried out in an air-opened system to investigate the effect of static and shaken culture, initial dye concentration, pH, temperature, salinity, carbon source, nitrogen source and metal ion on the decolorization of orange G dye wastewater by strain P2 . Meanwhile, the toxicity test for the non-decolorizing dye and decolorizing products using plant seed germination method and antimicrobial test were also conducted. The results showed that, compared with shaken culture, static culture was more suitable for decolorization by C. lacerata P2, and the optimal pH and temperature were 9 and 25 ℃, respectively. The results of salinity test showed that the decolorization rate of orange G was above 70% in 128 g L^-1 salt solution. Based on the system with optimal parameters, carbon source, nitrogen source and metal ion were separately added for choosing appropriate factors. The results indicated that the optimal condition for decolorization were as follows： 4 g L^-1 glucose, 0.15 g L^-1 ammonium nitrate and 0.1 mmol L^-1 zinc ion. The results of biosorption test showed that mycelial sorption played a minor role in decolorization, and the decolorization was primarily driven by the degrading enzymes. In this study, laccase activity was not detected, while only manganese peroxidase and lignin peroxidase were checked with a maximum activity of 230 U mL^-1 and 158 U mL^-1, respectively. The results of toxicity test suggested that, in comparison with non-decolorized dye, toxicity of decolorized solution on plant seed germination and microbial organism greatly declined. Therefore, C. lacerata P2 had a good application potential in treating orange G dye wastewater. Fig 6, Tab 1, Ref 27
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2011年第6期876-882,共7页
Chinese Journal of Applied and Environmental Biology
基金
湖南省教育厅项目(No.10C1118)
湖南省自然科学基金项目(No.10JJ6053)
湖南省科技厅项目(No.2009FJ3041)
国家自然科学基金项目(No.40971151)
吉首大学科研基金(Nos.jsdxkyzz200901
10JD028)~~
关键词
撕裂蜡孔菌
脱色
橙黄G
染料废水
酶活性
毒性测试
Ceriporia lacerata
decolorization
orange G
dye wastewater
enzyme activity
toxicity test
