摘要
采用灌喂方法,研究不同剂量磺胺二甲氧嘧啶钠(SMS)100、200、400mg/kg对斑点叉尾鮰门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、碱性磷酸酶(ALP)、乳酸脱氢酶(LDH)、肌酐(CRE)、尿素(UREA)等血清生化指标以及肝脏和肾脏组织的影响。结果表明:连续灌药5d后,对照组与给药组斑点叉尾鮰血清中的AST、ALT、ALP、LDH和UREA均无显著性变化,按400mg/kg体质量给药组的肌酐存在显著性变化(P<0.05)。连续灌药10d后,与对照组相比,给药组AST、ALT、ALP、LDH、CRE、UREA均有显著性变化。SMS可导致肝细胞核溶解或细胞溶解,肾脏肾小管上皮细胞局部坏死,且浓度越高,损害越大。试验表明,100mg/kg的剂量对斑点叉尾鮰在较长时间的使用过程中毒副作用较小,在5d内使用是安全的;200mg/kg的剂量在较短时间内使用是安全的,但是不能长时间使用,否则会对肝脏及肾脏造成损伤;而高剂量的400mg/kg对斑点叉尾鮰在较短时间内仍具有较大的毒副作用,表现在肾脏上的损伤。
Effects of dose administration（perfused at 100,200,400 mg/kg）of sulfadimethoxine sodi- um on the serum biochemical parameters including aspartate aminotransferase （AST）, alanine amin- otransferase （ALT）, alkalinephoshatase （ALP）, lactate dehydrogenase （LDH）, creatinine （CRE） and urea （UREA）,and the structure of liver and kidney of channel catfish （Ictalurus punctatus ） were stud- ied. No differences were found in the activities of AST, ALT, ALP, LDH and the content of UREA be- tween the control group and the treated groups after consecutively perfused for 5 days. But the content of CRE increased significantly at the dose of 400 mg/kg. After consecutively perfused for 10 days, the activ- ites of AST, ALT, ALP and LDH, and the contents of CRE and UREA changed obviously among the groups. From the tissue slices of liver and kidney in the 10th day, the livers and kidneys were damaged by SMS, inducing the cell lysis of liver and epithelial cell of tubular necrosed, and the higher the concentra- tion, the greater the damage. Therefore, after long-term use of 100 mg/kg SMS, there was less toxic and side effects, and it was safe in 5 days. The dose of 200 mg/kg SMS was safe in a short time, otherwise the structure of liver and kidney would be damaged. The high dose of SMS had toxic damage on the structure of kidney in a short time.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2012年第1期112-115,共4页
Journal of Huazhong Agricultural University
基金
湖北省自然科学基金(2009CDB230)
现代农业产业技术体系建设专项资金(nycytx-49-23)
