摘要
目的探讨大肠癌微血管密度(MVD)及增殖细胞核抗原(PCNA)与手术后有无潜在性肿瘤转移及复发的相关性。方法对55例大肠癌进行术后5a的随访及石蜡标本的S-P免疫组化法染色。结果大肠癌MVD与其分化程度密切相关(P<0.01);与临床病理分期(Dukes')间差异有显著意义(P<0.05);与有无淋巴结、肝转移密切相关(P<0.05);在术后复发与无复发生存者间差异有非常显著性意义(F<0.01)。增殖活性表达提示,分化愈差,有淋巴结或肝转移时,增殖活性增高,术后复发与无复发生存者之间,增殖活性差异有显著意义(P<0.05)。结论大肠癌MVD及PCNA与肿瘤的浸润、淋巴结及肝转移相关。手术时虽无明显转移,但MVD增高及PCNA活性增强,提示可能有潜在的转移存在。
AIM To investigate microvessel density (MVD) and proliferating cell nuclear antigen (PCNA) in colorectal cancer and its relationship with metastasis and recurrence.METHODS Paraffin-embedded specimens from 55 patients with colorectal cancer were examined by SP immunohistochemical method using monoclonal antigen (F-ⅧAg) and PCNA.RESULTS MVD was significantly correlated with differentiation (P<0.01). It was significantly different from clinical stage (Dukes stage) and to lymph node or liver metastasis (P<0.05). It was remarkably different between the cases with recurrence or without (P<0.01). But the expression of PCNA showed that the poorer the differentiation the higher intensity of expression was, the PCNA increased in cases with metastasis of lymph node or liver, and was significantly different between the cases with postoperational recurrence or without (P<0.05). CONCLUSION MVD and PCNA were related to invasion and metastasis of lymph node or liver. Although there was no obvious metastasis of lymph node and liver during the operation, the increased MVD and PCNA implied that there were invasion or subsets of metastasis, which were risk factors for recurrence and metastasis.
出处
《世界华人消化杂志》
CAS
2000年第1期74-76,共3页
World Chinese Journal of Digestology
关键词
微血管密度
增殖细胞核抗原
大肠癌
colorectal neoplasms
microvessel density
proliferating cell nuclear antigen
prognosis
