摘要
:目的 应用改良的随机引物聚合酶链反应(AP-PCR)技术,分离配对的食管癌组织和非癌食管组织中差异基因片断,并进行克隆、测序和序列分析。方法 在来自食管癌高发区的22例配对的食管癌组织和非癌食管组织中,应用AP-PCR技术测检了差异基因片段。结果 22例配对食管癌组织和非癌食管组织中,6例癌组织中有差异随机扩增片断,而相应的非癌组织缺如。其中5T差异基因片断为10kb左右,经克隆、测序和序列的同源性分析,基因文库内无同源序列。结论 5T差异基因片断是否为一个新的侯选基因或癌基因标志物,有待进一步筛查。
Objective The authors present that preliminary results of isolation of gene difference fragments from matched pairs of esophageal canaer tissue and noncancer tissue. And the fragments have been carried out cloning, sequencing and sequencing analysis. Method Useing the application of related gene was examined with improved AP-PCR among of 22 cases from the high-risk population for esophageal cancer of matched pairs of the cancerous and noncancerous tissue. Results Among of 22 cases of matched pairs of the cancerous and noncancerous tissue there are 6 cases showing randomly amplified fragment of gene difference in cancer tissue,and none in noncancer. Among them the 5T fragment of difference gene was about 1 0 kb and failed to find homologous sequence in gene bank. Conclusion The 5T fragment of gene difference may be a new oncogen or cancer marker.
出处
《河南肿瘤学杂志》
2000年第1期1-2,共2页
Henan Journal of Oncology
关键词
食管肿瘤
DNA指纹图
聚合酶链反应
esophageal cancer
DNA fingerprinting
polymerase chain reaction
