摘要
目的探讨结直肠癌病理分期、组织分型及手术治疗对血清Th1/Th2细胞因子变化的不同影响及其临床意义。方法采用双抗体夹心酶联免疫吸附法(ELISA)检测45例结直肠癌患者和10例健康体检者(正常对照组)外周血中干扰素γ(IFN-γ)、白介素(IL)-2、IL-4、IL-10的水平。结果正常对照组、Dukes'B组、Dukes'C组和Dukes'D组IFN-γ、IL-2、IL-4和IL-10水平比较,差异有统计学意义(P<0.01)。其中Dukes'B组、Dukes'C组和Dukes'D组较正常对照组IFN-γ、IL-2明显降低,IL-4、IL-10明显升高,差异均有统计学意义(P<0.05);结直肠癌患者随着Dukes'分期的升高,IFN-γ、IL-2逐渐降低,IL-4、IL-10逐渐升高,差异均有统计学意义(P<0.05)。腺癌组和黏液性腺癌组IFN-γ、IL-2、IL-4和IL-10水平比较,差异无统计学意义(P>0.05)。术后较术前IFN-γ、IL-2明显升高,IL-4、IL-10明显降低,差异均有统计学意义(P<0.01)。但术后较正常对照组IFN-γ、IL-2明显降低,IL-4、IL-10明显升高,差异均有统计学意义(P<0.01)。结论结直肠癌患者外周血中Th1、Th2免疫反应失衡,向Th2方向漂移;结直肠癌患者外周血Th1/Th2失衡程度与临床分期呈正相关,与肿瘤的组织分型无关;术后Th1/Th2失衡有可能逆转。
Objective To investigate the influence of pathological staging, tissue - typing and operation on Th1/Th2 imbalance in colorectal cancer patients and its significance through detecting the serum levels of Th1/Th2 type cytokines. Methods 45 colorectal cancer patients and 10 healthy volunteers （control group） were recruited for the study. Peripheral blood samples were collected, and the levels of IFN - γ, IL - 2, IL - 4, IL - 10 levels were detected by ELISA kits. Results The differences of the levels of IFN - γ, IL - 2, IL - 4 and IL - 10 between control group, Dukes＇B group, Dukes＇C group and Dukes＇D group were significant （P 〈0. 01 ） . Compared with control group, IL -2 and IFN -γ levels of colorectal cancer patients were significantly decreased （P 〈 0. 05 ）, but IL -4, IL - 10 levels were significantly increased （P 〈 0. 05 ） . With pathogenetic condition aggravating, IL -4 and IL - 10 levels were increased gradually, while IL -2 and IFN - γ levels were decreased gradually, and the difference was statistically significant （P 〈 0. 05） . The cytokine levels were not significantly different between adenocarcinoma patients and mueinous adenocarcinoma patients （P 〉 0. 05） . After operation, IL - 2 and IFN - γ levels were increased significantly （P 〈0. 01 ）, but IL- 4 and IL- 10 levels were decreased significantly （P 〈 0. 01 ） . After operation, compared with control group, the IFN-~/and IL-2 levels were significantly decreased, while IL-4 and IL-l0 levels were significantly increased （P 〈0. 01 ）. Conclusion There is response imbalance in Thl and Th2 in colorectal cancer patients. The imbalanced Th1/Th2 response is correlated with Dukes staging, but uncorrelated with tissuetyping, and can be reversed by Operation.
出处
《中国全科医学》
CAS
CSCD
北大核心
2011年第36期4136-4138,共3页
Chinese General Practice
关键词
结直肠肿瘤
T淋巴细胞
肿瘤分期
组织分型
Colorectal neoplasms
T - lymphocytes
Neoplasms staging
Tissue - typing
